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Titolo:
Suppression of IL-8 gene expression by radicicol is mediated through the inhibition of ERK1/2 and p38 signaling and negative regulation of NF-kappa Band AP-1
Autore:
Na, YJ; Jeon, YJ; Suh, JH; Kang, JS; Yang, KH; Kim, HM;
Indirizzi:
Korea Res Inst Biosci & Biotechnol, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 South Korea Korea Adv Inst Sci & Technol, Taejon 305701, South Korea Korea Adv Inst Sci & Technol Taejon South Korea 305701 5701, South Korea
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 9-10, volume: 1, anno: 2001,
pagine: 1877 - 1887
SICI:
1567-5769(200109)1:9-10<1877:SOIGEB>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; NITRIC-OXIDE SYNTHASE; INTERLEUKIN-8 GENE; EPITHELIAL-CELLS; ENDOTHELIAL-CELLS; MONOCYTIC CELLS; TRANSCRIPTION; LIPOPOLYSACCHARIDE; NEUTROPHIL; CYTOKINES;
Keywords:
radicicol; interleukin-8; ERK1/2; p38; NF-kappa B; AP-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, HM Korea Res Inst Biosci & Biotechnol, POB 115, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol POB 115 Taejon South Korea 305600
Citazione:
Y.J. Na et al., "Suppression of IL-8 gene expression by radicicol is mediated through the inhibition of ERK1/2 and p38 signaling and negative regulation of NF-kappa Band AP-1", INT IMMUNO, 1(9-10), 2001, pp. 1877-1887

Abstract

We show that radicicol, an anti-fungal agent, inhibits interleukin-8 (IL-8) production by the human monocyte line THP-1 in response to phorbol-12-myristate-13- acetate/lipopolysaccharide (PMA/LPS). IL-8 is a potent chemokineand needs for an optimal immune response-such as inflammation by activation of neutrophils. The decrease in PMA/LPS-induced IL-8 mRNA expression was demonstrated by quantitative reverse transcription-polymerase chain reaction (RT-PCR). Since the promoter in IL-8 gene contains binding motifs for NF-kappaB, AP-1, and NF-IL-6, which appear to be important in IL-8 induction, the effects of radicicol on the activation of these transcription factors were examined. Treatment of radicicol to THP-I cells produced a strong inhibition of NF-kappaB and AP-1, while NF-IL6 was not significantly affected byradicicol. Western blot analysis showed that radicicol inhibited the phosphorylation and phosphotransferase activities of extracellular signal-regulated kinases I and 2 (ERK1/2) and p38. PD98059 and SB203580, known as a specific inhibitor of MEK1 and p38 kinase, respectively, inhibited IL-8 gene expression showing that both of the kinase pathways are involved in IL-8 regulation in human monocytes, Collectively, this series of experiments indicates that radicicol inhibits IL-8 gene expression by blocking ERK1/2 and p38 signaling. (C) 2001 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:52:07