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Titolo:
The effects of triodothyronine and thymulin on avian NK cytolytic activity
Autore:
Marsh, JA; Merlino, PG; Staeheli, P;
Indirizzi:
Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, Ithaca, NY 14853 USACornell Univ Ithaca NY USA 14853 icrobiol & Immunol, Ithaca, NY 14853 USA Univ Freiburg, Abt Virol, Inst Med Microbiol & Hyg, D-79008 Freiburg, Germany Univ Freiburg Freiburg Germany D-79008 & Hyg, D-79008 Freiburg, Germany
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 9-10, volume: 1, anno: 2001,
pagine: 1823 - 1830
SICI:
1567-5769(200109)1:9-10<1823:TEOTAT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
KILLER CELL-ACTIVITY; THYROID-HORMONE TREATMENT; RECEPTOR EXPRESSION; GROWTH-HORMONE; OLD MICE; INTERLEUKIN-2; MODULATION; CHICKENS; TRIIODOTHYRONINE; LYMPHOCYTES;
Keywords:
triiodothyronine; thymulin; avian interferon gamma; natural killer cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Marsh, JA Cornell Univ, Coll Vet Med, Dept Microbiol & Immunol, C5-103 VMC, Ithaca, NY 14853 USA Cornell Univ C5-103 VMC Ithaca NY USA 14853 Ithaca, NY 14853 USA
Citazione:
J.A. Marsh et al., "The effects of triodothyronine and thymulin on avian NK cytolytic activity", INT IMMUNO, 1(9-10), 2001, pp. 1823-1830

Abstract

The effects of triiodothyronine (T3), thymulin, and recombinant chicken interferon-gamma (ChIFN-gamma) on natural killer (NK) cell cytotoxicity was investigated using the euthyroid control K and the T3-deficient sex-linked dwarf (SLD) chicken strains. Factorial design experiments were used to investigate the effects of T3 treatments where animals of both strains received either 0 or 0.1 ppm T3 supplementation to the standard chick starter diet. The ChIFN-gamma treatments were administered in vitro by incubation with effector cells overnight prior to the addition of the RP9 lymphoblastoma target cell line. All cytotoxicity assays were run at 50:1 and 25:1 effector/target (E/T) ratios. Treatments were begun at hatching and continued through 7 weeks. NK cells for these assays were enriched by separation of splenocytes over ficoll. Splenocyte preparations from untreated K strain consistently had significantly higher NK-mediated cytolysis than did samples from the untreated SLD at both E/T ratios. T3 treatment alone had no effect on NK activity in cell preparations from the K strain but did significantly enhancethat activity in the T3-deficient SLD whereas IFN treatment alone enhancedNK activity in both strains. The combined T3 and IFN treatments resulted in a greater enhancement of NK cytolytic activity in both strains than any separate treatment and resulted in an elimination of differences in NK cell responsiveness between the K and SLID strains. Similar results were obtained when NK cell cultures were incubated in vitro with thymulin prior to assessing cytotoxicity. In vitro thymulin treatments alone significantly enhanced cytolytic activity for NK cells for both K and SLD strains. The greatesteffect of in vitro thymulin exposure was to increase the responsiveness toNK cells to ChIFN-gamma stimulation. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 26/01/20 alle ore 00:49:33