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Titolo:
Anti-inflammatory effects of chemically modified tetracyclines by the inhibition of nitric oxide and interleukin-12 synthesis in J774 cell line
Autore:
DAgostino, P; Ferlazzo, V; Milano, S; La Rosa, M; Di Bella, G; Caruso, R; Barbera, C; Grimaudo, S; Tolomeo, M; Feo, S; Cillari, E;
Indirizzi:
V Cervello Hosp, Palermo, Italy V Cervello Hosp Palermo ItalyV Cervello Hosp, Palermo, Italy Univ Palermo, Dept Biopathol & Biomed Methodol, Palermo, Italy Univ Palermo Palermo Italy Biopathol & Biomed Methodol, Palermo, Italy Univ Palermo, Dept Immunohaematol & Transfus, Palermo, Italy Univ PalermoPalermo Italy pt Immunohaematol & Transfus, Palermo, Italy V Cervello Hosp, Clin Pathol & Microbiol Lab, I-90142 Palermo, Italy V Cervello Hosp Palermo Italy I-90142 robiol Lab, I-90142 Palermo, Italy Univ Palermo, Dept Cellular & Dev Biol, Palermo, Italy Univ Palermo Palermo Italy mo, Dept Cellular & Dev Biol, Palermo, Italy
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 9-10, volume: 1, anno: 2001,
pagine: 1765 - 1776
SICI:
1567-5769(200109)1:9-10<1765:AEOCMT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE MACROPHAGES; LETHAL ENDOTOXEMIA; SYNTHASE; MECHANISM; DOXYCYCLINE; APOPTOSIS; SURVIVAL; DISEASE;
Keywords:
chemically modified tetracyclines; nitric oxide; interleukin-12;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Cillari, E V Cervello Hosp, Palermo, Italy V Cervello Hosp Palermo Italy Cervello Hosp, Palermo, Italy
Citazione:
P. D'Agostino et al., "Anti-inflammatory effects of chemically modified tetracyclines by the inhibition of nitric oxide and interleukin-12 synthesis in J774 cell line", INT IMMUNO, 1(9-10), 2001, pp. 1765-1776

Abstract

We investigated the effects of chemically modified tetracyclines (CMTs) onthe production of nitric oxide (NO) and on the synthesis of some cytokines: turnout necrosis factor alpha (TNF-alpha), interleukin(IL)-10 and IL-12 in lipopolysaccharide (LPS)-treated J774 cell line. Furthermore, we studied the ability of these drugs to modify the viability in LPS-stimulated J774 macrophages. CMTs decreased, in a dose-dependent manner, inducible NO synthase (iNOS) activity and, consequently, nitrite formation in J774 cultures. The CMT-induced decrease in NO production is due to the inhibition of enzymeactivity rather than to a direct effect on enzyme expression. The absence of the inhibition in mRNA accumulation indicates that the inhibiting activity is mainly post-transcriptional. CMTs were unable to modulate TNF-alpha and IL-10 synthesis and they were not effective in modifying the transcription of relative mRNA in J774 macrophages. On the contrary, IL-12 mRNA expression was significantly increased by CMT-1 and CMT-8 with LPS activation. Since IL-12 protein secretion was inhibited by CMTs, these compounds interfere in the blocking of post-transcriptional events. The studies on cell viability showed that various CMTs induced a dose-dependent decrease in J774 macrophage viability. The cytotoxic activity was present even though NO production was inhibited by CMTs. These compounds appear to be able to activate apoptosis in aNO-independent way. Altogether, these results indicate that CMTs can exert anti-inflammatory effects by inhibiting NO synthesis. and theyare able to modify cell viability by exerting a strong apoptotic activity. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:01:06