Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Increased histidine decarboxylase expression during in vitro monocyte maturation; a possible role of endogenously synthesised histamine in monocyte/macrophage differentiation
Autore:
Laszlo, V; Rothe, G; Hegyesi, H; Szeberenyi, JB; Orso, E; Schmitz, G; Falus, A;
Indirizzi:
Semmelweis Univ Med, Dept Genet Cell & Immunobiol, H-1089 Budapest, Hungary Semmelweis Univ Med Budapest Hungary H-1089 ol, H-1089 Budapest, Hungary Univ Regensburg, Inst Clin Chem & Lab Med, D-8400 Regensburg, Germany UnivRegensburg Regensburg Germany D-8400 ed, D-8400 Regensburg, Germany Hungarian Acad Sci, Mol Immunol Res Grp, Budapest, Hungary Hungarian Acad Sci Budapest Hungary Immunol Res Grp, Budapest, Hungary
Titolo Testata:
INFLAMMATION RESEARCH
fascicolo: 8, volume: 50, anno: 2001,
pagine: 428 - 434
SICI:
1023-3830(200108)50:8<428:IHDEDI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; INTRACELLULAR HISTAMINE; FLOW-CYTOMETRY; T-CELLS; MACROPHAGES; RECEPTORS; INTERLEUKIN-3; ANTAGONIST; MESSENGER; CYTOKINES;
Keywords:
monocyte; histamine; differentiation; M-CSF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Falus, A Semmelweis Univ Med, Dept Genet Cell & Immunobiol, Nagyvarad Ter 4, H-1089Budapest, Hungary Semmelweis Univ Med Nagyvarad Ter 4 Budapest Hungary H-1089 gary
Citazione:
V. Laszlo et al., "Increased histidine decarboxylase expression during in vitro monocyte maturation; a possible role of endogenously synthesised histamine in monocyte/macrophage differentiation", INFLAMM RES, 50(8), 2001, pp. 428-434

Abstract

Objective: In this study the expression of histidine decarboxylase (HDC), the pivotal enzyme in histamine formation and the effect of endogenously produced histamine on differentiation antigens was examined during in vitro differentiation of human monocytes. Material and treatment., Human elutriated monocytes from healthy volunteers were incubated with macrophage colony stimulating factor (M-CSF) and the expression of HDC was followed at both mRNA and protein levels. To study the possible function of histamine we followed the expression of some cell surface markers (CD14, CD16, CD91, CD49d and CD11c) relevant for phagocytic differentiation upon incubation in the presence of different histamine inhibitors, an HDC inhibitor: S(+)-alpha -fluoromethyl-histidine HCl, (alpha FMH), a compound that disturbs the interaction of histamine with intracellularcyp450 moieties: N,N-diethyl-2-[4-(phenyl-methyl) phenoxy]-ethanamine HCl. (DPPE); and H-1 and H-2 receptor antagonists, Triprolidine and Cimetidine. Results: During in vitro culture of elutriated human monocytes, in the presence of M-CSF, the gene expression and biosynthesis of HDC was considerably increased. The various antihistamine agents decreased th expression of the cell surface markers examined in this study. Conclusions:These data support the elevation of HDC expression during human monocytic differentiation and the possibility that monocyte-derived histamine is partially involved in regulation of M-CSF induced in vitro human monocyte/macrophage phagocytic differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/11/20 alle ore 20:11:04