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Titolo:
In vitro effects of aceclofenac and its metabolites on the production by chondrocytes of inflammatory mediators
Autore:
Henrotin, Y; de Leval, X; Mathy-Hartet, M; Mouithys-Mickalad, A; Deby-Dupont, G; Dogne, JM; Delarge, J; Reginster, JY;
Indirizzi:
CHU Sart Tilman, Bone & Cartilage Metab Res Unit, Inst Pathol, B-4000 Liege, Belgium CHU Sart Tilman Liege Belgium B-4000 Inst Pathol, B-4000 Liege, Belgium CHU Sart Tilman, Inst Pathol, Dept Med Chem, B-4000 Liege, Belgium CHU Sart Tilman Liege Belgium B-4000 ept Med Chem, B-4000 Liege, Belgium Inst Chem, Ctr Oxygen Res & Dev, B-4000 Liege, Belgium Inst Chem Liege Belgium B-4000 r Oxygen Res & Dev, B-4000 Liege, Belgium
Titolo Testata:
INFLAMMATION RESEARCH
fascicolo: 8, volume: 50, anno: 2001,
pagine: 391 - 399
SICI:
1023-3830(200108)50:8<391:IVEOAA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHEUMATOID SYNOVIAL-CELLS; 4'-HYDROXY ACECLOFENAC; ARTHRITIS; OSTEOARTHRITIS; EFFICACY; CYCLOOXYGENASE; TOLERABILITY; MULTICENTER; SUPPRESSES; DICLOFENAC;
Keywords:
inflammation; arthritis; chondrocytes; cytokines; nitric oxide; prostaglandins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Henrotin, Y CHU Sart Tilman, Bone & Cartilage Metab Res Unit, Inst Pathol,B-4000 Liege, Belgium CHU Sart Tilman Liege Belgium B-4000 , B-4000 Liege, Belgium
Citazione:
Y. Henrotin et al., "In vitro effects of aceclofenac and its metabolites on the production by chondrocytes of inflammatory mediators", INFLAMM RES, 50(8), 2001, pp. 391-399

Abstract

Objectives: To investigate the mechanisms of action underlying the anti-inflammatory action of aceclofenac in vivo, we studied in vitro the effect ofaceclofenac and its main metabolite, 4'-hydroxyaceclofenac, in comparison with diclofenac, another metabolite, on cyclooxygenases activity as well asinterleukin-1 beta, -6 and -8, nitric oxide, and prostaglandin E-2 production by human osteoarthritic and normal articular chondrocytes. Methods: Enzymatically isolated human chondrocytes were cultured for 72 Itin the absence or presence of interteukin-1 beta (IL-1 beta) or lipopolysacharride (LPS) and with or without increased amounts (1 to 30 muM) of aceclofenac or metabolites. The production of different cytokines was measured by Enzyme Amplified Sensitivity Immunoassays (EASIA). Prostaglandin E-2 was quantified by a specific radioimmunoassay. Nitrate and nitrate concentrations in the culture supernatants were determined by spectrophotometric methodbased upon the Griess reaction. Cyclooxygenase-2, inducible NO synthase and IL-1 beta gene expression were quantified by reverse transcription of mRNA followed by real time and quantitative polymerase chain reaction. Finally, cyclooxygenase inhibitory potency of the drugs was also tested in both a cell-free system using purified ovine cyclooxygenase-1 and -2 (COX-1 and COX-2) and at a cellular level using human whole blood assay.

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Documento generato il 04/04/20 alle ore 15:06:19