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Titolo:
Light-based imaging of green fluorescent protein-positive ovarian cancer xenografts during therapy
Autore:
Chaudhuri, TR; Mountz, JM; Rogers, BE; Partridge, EE; Zinn, KR;
Indirizzi:
Univ Alabama, Dept Radiol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ept Radiol, Birmingham, AL 35294 USA Univ Alabama, Dept Radiat Oncol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 diat Oncol, Birmingham, AL 35294 USA Univ Alabama, Dept Obstet & Gynecol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 & Gynecol, Birmingham, AL 35294 USA
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 3, volume: 82, anno: 2001,
pagine: 581 - 589
SICI:
0090-8258(200109)82:3<581:LIOGFP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON-EMISSION-TOMOGRAPHY; EXPRESSION; SCINTIMAMMOGRAPHY; ULTRASOUND; DIAGNOSIS; ONCOLOGY;
Keywords:
imaging; ovarian cancer; adriamycin; green fluorescent protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Chaudhuri, TR Univ Alabama, Dept Radiol, JT N333,619 19th St S, Birmingham, AL 35294 USA Univ Alabama JT N333,619 19th St S Birmingham AL USA 35294 A
Citazione:
T.R. Chaudhuri et al., "Light-based imaging of green fluorescent protein-positive ovarian cancer xenografts during therapy", GYNECOL ONC, 82(3), 2001, pp. 581-589

Abstract

Objective. The purpose of the study was to develop a sensitive, noninvasive imaging method for monitoring ovarian xenografts during therapeutic intervention. Methods. Human ovarian tumor cells (SKOV3.ip1) were infected with a replication-deficient adenoviral (Ad) vector encoding green fluorescent protein (GFP). The GFP-positive tumor cells were imaged in vitro and in vivo with a fluorescence stereomicroscope. Using appropriate filters, both GFP fluorescence and adriamycin were simultaneously detected. Nude mice implanted with GFP-positive cells were imaged repeatedly, in a noninvasive manner. Results. SKOV3.ip1 cells infected with Ad-GFP showed high GFP fluorescence, which was eliminated after treatment with adriamycin. Loss of GFP fluorescence was confirmed to be dead cells. For in vivo imaging, intraperitoneal tumors as small as 0.2 mm in diameter were detected externally. Adriamycin uptake was detected in tumors by in vivo imaging, and reduction in tumor size was concurrent with decrease in GFP fluorescence. These findings were confirmed at necropsy. Conclusions. Fluorescence stereomicroscopy monitored the response of ovarian xenografts to adriamycin therapy. For the first time, GFP and adriamycinwere imaged simultaneously. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 02:36:36