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Titolo:
Shotgun optical maps of the whole Escherichia coli O157 : H7 genome
Autore:
Lim, A; Dimalanta, ET; Potamousis, KD; Yen, G; Apodoca, J; Tao, CH; Lin, JY; Qi, R; Skiadas, J; Ramanathan, A; Perna, NT; Plunkett, G; Burland, V; Mau, B; Hackett, J; Blattner, FR; Anantharaman, TS; Mishra, B; Schwartz, DC;
Indirizzi:
Univ Wisconsin, Lab Mol & Computat Genom, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Computat Genom, Madison, WI 53706 USA Univ Wisconsin, Dept Chem, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Dept Chem, Madison, WI 53706 USA NYU, WM Keck Lab Biomol Imaging, New York, NY 10003 USA NYU New York NY USA 10003 Keck Lab Biomol Imaging, New York, NY 10003 USA Univ Wisconsin, Genet Lab, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Genet Lab, Madison, WI 53706 USA Univ Wisconsin, Dept Biostat & Med Informat, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 & Med Informat, Madison, WI 53706 USA NYU, Courant Inst Math Sci, Dept Comp Sci, New York, NY 10012 USA NYU NewYork NY USA 10012 Math Sci, Dept Comp Sci, New York, NY 10012 USA Univ Wisconsin, Dept Genet, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 in, Dept Genet, Madison, WI 53706 USA
Titolo Testata:
GENOME RESEARCH
fascicolo: 9, volume: 11, anno: 2001,
pagine: 1584 - 1593
SICI:
1088-9051(200109)11:9<1584:SOMOTW>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY-ELECTROPHORESIS; ARRAY ELECTROPHORESIS; SEQUENCE; CLONES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartz, DC Univ Wisconsin, Lab Mol & Computat Genom, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 m, Madison, WI 53706 USA
Citazione:
A. Lim et al., "Shotgun optical maps of the whole Escherichia coli O157 : H7 genome", GENOME RES, 11(9), 2001, pp. 1584-1593

Abstract

We have constructed Nhe1 and Xho1 optical maps of Escherichia coil O157:H7solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coil O157:H7 is a common pathogen found in contaminated food and water. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, because maps were constructed from ensembles of single DNA molecules. Shotgun sequencingof bacterial genomes remains labor-intensive, despite advances in sequencing technology. This is partly due to manual intervention required during the last stages of finishing. The applicability of optical mapping to this problem was enhanced by advances in machine vision techniques that improved mapping throughput and created a path to full automation of mapping. Comparisons were made between maps and sequence data that characterized sequence gaps and guided nascent assemblies.

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Documento generato il 01/10/20 alle ore 06:27:51