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Titolo:
Expression of ribosome modulation factor (RMF) in Escherichia coli requires ppGpp
Autore:
Izutsu, K; Wada, A; Wada, C;
Indirizzi:
Kyoto Univ, Inst Virus Res, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 Virus Res, Sakyo Ku, Kyoto 6068507, Japan Osaka Med Coll, Dept Phys, Takatsuki, Osaka 5690084, Japan Osaka Med CollTakatsuki Osaka Japan 5690084 atsuki, Osaka 5690084, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 8, volume: 6, anno: 2001,
pagine: 665 - 676
SICI:
1356-9597(200108)6:8<665:EORMF(>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGMA-FACTOR SIGMA(S); PROTEIN H-NS; RNA-POLYMERASE; GUANOSINE TETRAPHOSPHATE; GEL-ELECTROPHORESIS; CELL VIABILITY; BINDING DOMAIN; GENE; INVOLVEMENT; SUBUNIT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Wada, C Kyoto Univ, Inst Virus Res, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 s, Sakyo Ku, Kyoto 6068507, Japan
Citazione:
K. Izutsu et al., "Expression of ribosome modulation factor (RMF) in Escherichia coli requires ppGpp", GENES CELLS, 6(8), 2001, pp. 665-676

Abstract

Background: During the transition from the logarithmic to the stationary phase, 70S ribosomes are dimerized into the 100S form, which has no translational activity. Ribosome Modulation Factor (P-MF) is induced during the stationary phase and binds to the 50S ribosomal subunit, which directs the dimerization of 70S ribosomes. Unlike many other genes induced in the stationary phase, rmf transcription is independent of the sigma S. To identify the factors that regulate the growth phase-dependent induction of rmf, mutant strains deficient in global regulators were examined for lacZ expression directed by the rmf promoter. Results: Among mutants of defective global regulators, only ppGpp deficiency (reL4-spoT double mutant) drastically reduced the level of rmf transcription to less than 10% of that seen in the wild-type. Neither RMF nor 100S ribosomes were detected in this mutant in the stationary phase. rmf transcription correlated well with cellular ppGpp levels during amino acid starvation, IPTG induction of Ptrc-relA455 and in other mutants with artificially increased ppGpp levels. Although the growth rate also correlated inversely with both ppGpp levels and rmf transcription, the observation that the growth rates of the ppGpp-deficient and wild-type strains varied equivalently when grown on different media indicates that the link between rmf transcription and ppGpp levels is not a function of the growth rate. Conclusions: ppGpp appears to positively regulate rmf transcription, at least in vivo. Thus, RMF provides a novel negative translational control by facilitating the formation of inactive ribosome dimers (100S) under the stringent circumstances of the stationary phase.

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Documento generato il 23/09/20 alle ore 12:53:10