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Titolo:
The yeast Xrs2 complex functions in S phase checkpoint regulation
Autore:
DAmours, D; Jackson, SP;
Indirizzi:
Univ Cambridge, Wellcome Trust & Canc Res Campaign, Inst Canc & Dev Biol, Cambridge CB2 1QR, England Univ Cambridge Cambridge England CB2 1QR iol, Cambridge CB2 1QR, England Univ Cambridge, Dept Zool, Cambridge CB2 1QR, England Univ Cambridge Cambridge England CB2 1QR ool, Cambridge CB2 1QR, England
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 17, volume: 15, anno: 2001,
pagine: 2238 - 2249
SICI:
0890-9369(20010901)15:17<2238:TYXCFI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAND BREAK REPAIR; DNA-DAMAGE RESPONSE; SACCHAROMYCES-CEREVISIAE; ATAXIA-TELANGIECTASIA; BUDDING YEAST; IONIZING-RADIATION; GENE DELETION; PROTEIN; MRE11; REPLICATION;
Keywords:
Xrs2p complex; checkpoint; Nijmegen breakage syndrome; Mre11; ATM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Jackson, SP Univ Cambridge, Wellcome Trust & Canc Res Campaign, Inst Canc & Dev Biol, Cambridge CB2 1QR, England Univ Cambridge Cambridge England CB2 1QR ge CB2 1QR, England
Citazione:
D. D'Amours e S.P. Jackson, "The yeast Xrs2 complex functions in S phase checkpoint regulation", GENE DEV, 15(17), 2001, pp. 2238-2249

Abstract

The Nbs1 complex is an evolutionarily conserved multisubunit nuclease composed of the Mre11, Rad50, and Nbs1 proteins. Hypomorphic mutations in the NBS1 or MRE11 genes in humans result in conditions characterized by DNA damage sensitivity, cell cycle checkpoint deficiency, and high cancer incidence. The equivalent complex in the yeast Saccharomyces cerevisiae (Xrs2p complex) has been implicated in DNA double-strand break repair and in telomere length regulation. Here, we find that xrs2 Delta, mre11 Delta, and rad50 Delta mutants are markedly defective in the initiation of the intra-S phase checkpoint in response to DNA damage. Furthermore, the absence of a functional Xrs2p complex leads to sensitivity to deoxynucleotide depletion and to aninability to efficiently slow down cell cycle progression in response to hydroxyurea. The checkpoint appears to require the nuclease activity of Mre11p and its defect is associated with the abrogation of the Tel1p/Mec1p signaling pathway. Notably, DNA damage induces phosphorylation of both Xrs2p and Mre11p in a Te11p-dependent manner. These results indicate that the Te11p/ATM signaling pathway is conserved from yeast to humans and suggest that the Xrs2p/Nbs1 complexes act as signal modifiers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 08:50:10