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Titolo:
Effect of glutathione depletion on caspase-3 independent apoptosis pathwayinduced by curcumin in Jurkat cells
Autore:
Piwocka, K; Jaruga, E; Skierski, J; Gradzka, I; Sikora, E;
Indirizzi:
M Nencki Inst Expt Biol, Lab Mol Bases Aging, PL-02093 Warsaw, Poland M Nencki Inst Expt Biol Warsaw Poland PL-02093 , PL-02093 Warsaw, Poland Inst Nucl Chem & Technol, Dept Radiobiol & Hlth Protect, PL-03195 Warsaw, Poland Inst Nucl Chem & Technol Warsaw Poland PL-03195 PL-03195 Warsaw, Poland
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 5, volume: 31, anno: 2001,
pagine: 670 - 678
SICI:
0891-5849(20010901)31:5<670:EOGDOC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLULAR REDOX STATE; AK-5 TUMOR-CELLS; NF-KAPPA-B; LIPID-PEROXIDATION; LEUKEMIA-CELLS; RAT-LIVER; C-MYC; BCL-2; INHIBITION; LINES;
Keywords:
apoptosis; curcumin; glutathione; Jurkat cells; cytochrome c; caspase-3; Bcl-2; DNA fragmentation; free radicals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Sikora, E M Nencki Inst Expt Biol, Lab Mol Bases Aging, Pasteur 3, PL-02093 Warsaw, Poland M Nencki Inst Expt Biol Pasteur 3 Warsaw Poland PL-02093 Poland
Citazione:
K. Piwocka et al., "Effect of glutathione depletion on caspase-3 independent apoptosis pathwayinduced by curcumin in Jurkat cells", FREE RAD B, 31(5), 2001, pp. 670-678

Abstract

Curcumin, a yellow pigment from Curcuma longa, exhibits anti-inflammatory,antitumor, and antioxidative properties. Although its precise mode of action has not been elucidated so far, numerous studies have shown that curcumin may induce apoptosis in normal and cancer cells. Previously, we showed that in Jurkat cells curcumin induced nontypical apoptosis-like pathway, which was independent of mitochondria and caspase-3. Now we show that the inhibition of caspase-3 by curcumin, which is accompanied by attenuation of internucleosomal DNA fragmentation, may be due to elevation of glutathione, which increased in curcumin-treated cells to 130% of control. We have demonstrated that glutathione depletion does not itself induce apoptosis in Jurkat cells, though, it can release cytochrome c from mitochondria and caspase-3 from inhibition by curcumin, as shown by Western blot. The level of Bcl-2 protein was not affected by glutathione depletion even upon curcumin treatment. Altogether, our results show that in Jurkat cells curcumin prevents glutathione decrease, thus protecting cells against caspase-3 activation and oligonucleosomal DNA fragmentation. On the other hand, it induces nonclassical apoptosis via a Still-Unrecognized mechanism, which leads to chromatin degradation and high-molecular-weight DNA fragmentation. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 14:43:22