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Titolo:
Age-related increase in mitochondrial superoxide generation in the testosterone-producing cells of Brown Norway rat testes: relationship to reduced steroidogenic function?
Autore:
Chen, HL; Cangello, D; Benson, S; Folmer, J; Zhu, H; Trush, MA; Zirkin, BR;
Indirizzi:
Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Biochem & Mol Biol, Div Reprod Biol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205d Biol, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Environm Hlth Sci, Div Toxicol Sci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 ol Sci, Baltimore, MD 21205 USA
Titolo Testata:
EXPERIMENTAL GERONTOLOGY
fascicolo: 8, volume: 36, anno: 2001,
pagine: 1361 - 1373
SICI:
0531-5565(200108)36:8<1361:AIIMSG>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROSOMAL CYTOCHROME-P-450 ENZYMES; OXYGEN-MEDIATED DAMAGE; ANION-RADICAL PRODUCTION; BIS-N-METHYLACRIDINIUM; LEYDIG-CELLS; LUTEINIZING-HORMONE; LIPID-PEROXIDATION; OXIDATIVE STRESS; ADRENAL-CORTEX; DESENSITIZATION;
Keywords:
aging; testis; Leydig cells; mitochondrial superoxide generation; lucigenin; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, HL Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Biochem & Mol Biol,Div Reprod Biol, 615 N Wolfe St, Baltimore, MD 21205 USA Johns Hopkins Univ 615 N Wolfe St Baltimore MD USA 21205 1205 USA
Citazione:
H.L. Chen et al., "Age-related increase in mitochondrial superoxide generation in the testosterone-producing cells of Brown Norway rat testes: relationship to reduced steroidogenic function?", EXP GERONT, 36(8), 2001, pp. 1361-1373

Abstract

Aging in Brown Norway rats is accompanied by the reduced production of testosterone by the Leydig cells, the testicular cells responsible for synthesizing and secreting this essential steroid. As yet, the mechanism by which Leydig cell steroidogenesis is reduced is unknown. Herein we assess the production of mitochondrial reactive oxygen species by intact Leydig cells isolated from the testes of young and old rats. To this end, Leydig cells wereincubated with lucigenin (bis-N-methylacridinium. nitrate), a probe that enters cells, localizes to mitochondria, and yields a significant chemiluminescent response following its reaction with intramitochondrial superoxide. Leydig cells from old rats elicited significantly greater lucigenin-derivedcherniluminescence (LDCL) than those from young rats. Electron microscopicstereological analysis revealed that the absolute volume of mitochondria in the old cells was reduced from that in the young. These results, taken together, suggest that there are age-related changes in the production of reactive oxygen species by the mitochondria of Leydig cells, with those of oldLeydig cells producing significantly greater levels than those of young Leydig cells. The results are consistent with the proposal that mitochondrial-derived reactive oxygen may play a role in the irreversible decline in theability of old Leydig cells to produce testosterone. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 09:40:51