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Titolo:
Dimerization partners determine the activity of the Twist bHLH protein during Drosophila mesoderm development
Autore:
Castanon, I; Von Stetina, S; Kass, J; Baylies, MK;
Indirizzi:
Mem Sloan Kettering Canc Ctr, Sloan Kettering Inst, Program Mol Biol, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr New York NY USA 10021 New York, NY 10021 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 16, volume: 128, anno: 2001,
pagine: 3145 - 3159
SICI:
0950-1991(200108)128:16<3145:DPDTAO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOOP-HELIX PROTEINS; SAETHRE-CHOTZEN SYNDROME; SEX DETERMINATION GENE; ACHAETE-SCUTE; DNA-BINDING; TRANSCRIPTIONAL-ACTIVATION; MOUSE EMBRYOGENESIS; FGF-RECEPTOR; CELL FATES; NUCLEAR-PROTEIN;
Keywords:
muscle; myogenesis; daughterless; tethered dimers; Twist; Drosophila melanogaster;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
86
Recensione:
Indirizzi per estratti:
Indirizzo: Baylies, MK Mem Sloan Kettering Canc Ctr, Sloan Kettering Inst, Program Mol Biol, 1275York Ave, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY USA 10021
Citazione:
I. Castanon et al., "Dimerization partners determine the activity of the Twist bHLH protein during Drosophila mesoderm development", DEVELOPMENT, 128(16), 2001, pp. 3145-3159

Abstract

The basic helix-loop-helix transcription factor Twist regulates a series of distinct cell fate decisions within the Drosophila mesodermal lineage. These twist functions are reflected in its dynamic pattern of expression, which is characterized by initial uniform expression during mesoderm induction, followed by modulated expression at high and low levels in each mesodermal segment, and finally restricted expression in adult muscle progenitors. We show two distinct partner-dependent functions for Twist that are crucial for cell fate choice. We find that Twist can form homodimers and heterodimers with the Drosophila E protein homologue, Daughterless, in vitro. Using tethered dimers to assess directly the function of these two particular dimers in vivo, we show that Twist homodimers specify mesoderm and the subsequent allocation of mesodermal cells to the somatic muscle fate. Misexpressionof Twist-tethered homodimers in the ectoderm or mesoderm leads to ectopic somatic muscle formation overriding other developmental cell fates. In addition, expression of tethered Twist homodimers in embryos null for twist canrescue mesoderm induction as well as somatic muscle development. Loss of function analyses, misexpression and dosage experiments, and biochemical studies indicate that heterodimers of Twist and Daughterless repressgenes required for somatic myogenesis. We propose that these two opposing roles explain how modulated Twist levels promote the allocation of cells tothe somatic muscle fate during the subdivision of the mesoderm. Moreover, this work provides a paradigm for understanding how the same protein controls a sequence of events within a single lineage.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 22:52:46