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Titolo:
Comparative nuclear magnetic resonance studies of water permeability of red blood cells from maternal venous blood and newborn umbilical cord blood
Autore:
Benga, G; Frentescu, L; Matei, H; Tigan, S;
Indirizzi:
Luliu Hatieganu Univ Med & Pharm, Dept Cell & Mol Biol, Cluj Napoca 3400, Romania Luliu Hatieganu Univ Med & Pharm Cluj Napoca Romania 3400 3400, Romania Luliu Hatieganu Univ Med & Pharm, Dept Informat & Biostat, Cluj Napoca 3400, Romania Luliu Hatieganu Univ Med & Pharm Cluj Napoca Romania 3400 3400, Romania
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 7, volume: 39, anno: 2001,
pagine: 606 - 611
SICI:
1434-6621(200107)39:7<606:CNMRSO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ERYTHROCYTE-MEMBRANES; DIFFUSION PERMEABILITY; SULFHYDRYL-REAGENTS; AQUAPORIN-CHIP; TRANSPORT; EXCHANGE; INHIBITION; EXPRESSION; GHOSTS; GENE;
Keywords:
red blood cells; fetal red blood cells; water permeability; NMR = nuclear magnetic resonance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Benga, G Luliu Hatieganu Univ Med & Pharm, Dept Cell & Mol Biol, 6 PasteurStr, Cluj Napoca 3400, Romania Luliu Hatieganu Univ Med & Pharm 6 Pasteur Str Cluj Napoca Romania 3400
Citazione:
G. Benga et al., "Comparative nuclear magnetic resonance studies of water permeability of red blood cells from maternal venous blood and newborn umbilical cord blood", CLIN CH L M, 39(7), 2001, pp. 606-611

Abstract

Comparative morphological and nuclear magnetic resonance (NMR) measurements of the diffusional permeability (P-d) were performed on red blood cells (RBCs) from maternal venous blood and fetal RBCs, isolated from cord blood taken at delivery. Fetal RBC had a diameter of 8.79 +/- 0.03 pm (mean standard deviation, SD), a volume of 103 mum(3) and a surface area of 157 mum(2). We report here the first comparative measurements of Pd of maternal and fetal RBCs by using a Mn2+-doping NMR technique. The values of P-d were, in the case of maternal RBC, 3.7 x 10(-3) cm/s at 15 degreesC, 4.1 x 10(-3) cm/s at 20 degreesC, 4.9 x 10(-3) cm/s at 25 degreesC, 5.2 x 10(-3) cm/s at 30degreesC and 7.2 x 10(-3) cm/s at 37 OC. For fetal RBC all corresponding Pd values were almost half, namely 2.0 x 10(-3) cm/s at 15 degreesC, 2.3 x 10(-3) cm/s at 20 degreesC, 2.8 x 10(-3) cm/s at 25 degreesC, 3.4 x 10(-3) cm/s at 30 degreesC and 4.4 x 10(-3) cm/s at 37 degreesC. The decreased P-d values of fetal RBCs were probably due to lower channel-mediated water permeability compared with adult RBCs. The values of the activation energy for water permeability (E-a,E-d) were significantly higher for fetal RBCs (27.6+/- 5.0 kJ/mol) than for adult RBCs (22.8 +/- 2.7 kJ/mol). A positive correlation between the Pd values of the two kinds of RBCs was found. This points to the genetic basis for the determination of RBC water permeability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 20:49:24