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Titolo:
Distribution and depression of the GABA(B) receptor in the spinal dorsal horn of adult rat
Autore:
Yang, K; Wang, D; Li, YQ;
Indirizzi:
Fourth Mil Med Univ, Dept Anat, Xian 710032, Peoples R China Fourth Mil Med Univ Xian Peoples R China 710032 710032, Peoples R China Fourth Mil Med Univ, KK Leung Brain Res Ctr, Xian 710032, Peoples R China Fourth Mil Med Univ Xian Peoples R China 710032 710032, Peoples R China
Titolo Testata:
BRAIN RESEARCH BULLETIN
fascicolo: 4, volume: 55, anno: 2001,
pagine: 479 - 485
SICI:
0361-9230(20010701)55:4<479:DADOTG>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUBSTANTIA-GELATINOSA NEURONS; CORD IN-VITRO; IMMUNOREACTIVITY RELEASE; PRESYNAPTIC INHIBITION; SYNAPTIC TRANSMISSION; BACLOFEN; AFFERENT; (-)-BACLOFEN; TERMINALS; CAPSAICIN;
Keywords:
GABA; receptor; baclofen; synaptic transmission; spinal cord; slice; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Li, YQ Fourth Mil Med Univ, Dept Anat, Xian 710032, Peoples R China FourthMil Med Univ Xian Peoples R China 710032 , Peoples R China
Citazione:
K. Yang et al., "Distribution and depression of the GABA(B) receptor in the spinal dorsal horn of adult rat", BRAIN RES B, 55(4), 2001, pp. 479-485

Abstract

gamma -Aminobutyric acid (GABA) is a principal inhibitory neurotransmitterin vertebrate nervous system. The metabotropic receptor for GABA, GABA(B) receptor, is characterized as a G protein-coupled receptor subtype. In the present study, GABA(B) receptor-like immunoreactivity (GABA(B)R-LI) in the rat spinal cord and dorsal root ganglion (DRG), as well as GABA(B), receptor-mediated depression in the spinal dorsal horn were examined by using immunohistochemistry and whole-cell voltage-clamp recording technique, respectively. Under light microscope, GABA(B)R-LI was densely found in laminae I and II of the dorsal horn. DRG cells of various diameters also showed GABA(B)R-LI. Electron microscopy further revealed that GABA(B)R-LI was also localized in terminals of myelinated, unmyelinated fibers as well as the somatodendritic sites of dorsal horn neurons. Bath application of a GABA(B) receptor agonist, baclofen (10 muM, 30 s), induced a slow outward (inhibitory) current in dorsal horn neurons. This slow current was depressed when the postsynaptic G protein-coupled receptor was inhibited, indicating the postsynaptic action of baclofen. Under the condition of postsynaptic GABA(B) receptorbeing inhibited, baclofen (10 muM, 60 s) depressed large (A beta) and fine(C, A delta) afferent fiber-evoked monosynaptic excitatory postsynaptic currents, indicating presynaptic inhibition of GABA(B), receptor on elicited neurotransmitter release. Taken together, the results suggest that baclofen-sensitive GABA(B) receptor is expressed pre- and postsynaptically on primary afferent fibers and neurons in the spinal dorsal horn; activation of GABA(B) receptor in the dorsal horn postsynaptically hyperpolarizes dorsal horn neurons and presynaptically inhibits primary afferents. (C) 2001 ElsevierScience Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:23:14