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Titolo:
Polymerase chain reaction as a diagnostic adjunct in herpesvirus infections of the nervous system
Autore:
Kleinschmidt-Demasters, BK; DeBiasi, RL; Tyler, KL;
Indirizzi:
Univ Colorado, Hlth Sci Ctr, Dept Pathol B216, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Dept Pathol B216, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Dept Neurol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Ctr, Dept Neurol, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Ctr, Dept Pediat, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Dept Microbiol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 r, Dept Microbiol, Denver, CO 80262 USA Univ Colorado, Hlth Sci Ctr, Dept Immunol & Med, Denver, CO 80262 USA UnivColorado Denver CO USA 80262 ept Immunol & Med, Denver, CO 80262 USA Denver Vet Adm Hosp, Denver, CO USA Denver Vet Adm Hosp Denver CO USADenver Vet Adm Hosp, Denver, CO USA
Titolo Testata:
BRAIN PATHOLOGY
fascicolo: 4, volume: 11, anno: 2001,
pagine: 452 - 464
SICI:
1015-6305(200110)11:4<452:PCRAAD>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
VARICELLA-ZOSTER VIRUS; HUMAN-IMMUNODEFICIENCY-VIRUS; BRAIN-STEM ENCEPHALITIS; RAMSAY-HUNT-SYNDROME; SIMPLEX VIRUS; CEREBROSPINAL-FLUID; VIRAL-DNA; AIDS PATIENTS; ALZHEIMERS-DISEASE; CYTOMEGALOVIRUS GENOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
92
Recensione:
Indirizzi per estratti:
Indirizzo: Kleinschmidt-Demasters, BK Univ Colorado, Hlth Sci Ctr, Dept Pathol B216, 4200 E 9th Ave, Denver, CO 80262 USA Univ Colorado 4200 E 9th Ave Denver COUSA 80262
Citazione:
B.K. Kleinschmidt-Demasters et al., "Polymerase chain reaction as a diagnostic adjunct in herpesvirus infections of the nervous system", BRAIN PATH, 11(4), 2001, pp. 452-464

Abstract

Polymerase chain reaction (PCR) is a powerful technique that allows detection of minute quantities of DNA or RNA in cerebrospinal fluid (CSF), vesicle and endoneurial fluids, blood, fresh-frozen, and even formalin-fixed tissues. Various infectious agents can be detected with high specificity and sensitivity, including bacteria, parasites, rickettsia and viruses. PCR analysis of CSF has revolutionized the diagnosis of nervous system viral infections, particularly those caused by human herpesviruses (HHV), and has now replaced brain biopsy as the gold standard for diagnosis of herpes simplex virus (HSV) encephalitis. PCR analysis of both CSF and nervous system tissueshas also broadened our understanding of the spectrum of disease caused by HSV-1 and -2, cytomegalovirus (CMV), Epstein-Barr virus (EBV), varicella zoster virus (VZV), and HHV-6. Nonetheless, positive tissue PCR results must be interpreted cautiously, especially in cases that lack corroborating clinical and neuropathologic evidence of infection. Moreover, positive PCR results from tissues do not distinguish latent from productive (lytic) viral infections. In several neurological diseases, negative PCR results have provided strong evidence against a role for herpesviruses as the causative agents. This review focuses on the use of PCR tests to diagnose HSV and VZV infections of the nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 03:40:20