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Titolo:
A transformation system for an ectomycorrhizal basidiomycete, Lyophyllum shimeji
Autore:
Saito, T; Tanaka, N; Shinozawa, T;
Indirizzi:
Gunma Univ, Fac Engn, Dept Biol & Chem Engn, Kiryu, Gumma 3768515, Japan Gunma Univ Kiryu Gumma Japan 3768515 em Engn, Kiryu, Gumma 3768515, Japan Mushroom Res Inst Japan, Kiryu, Gumma 3760051, Japan Mushroom Res Inst Japan Kiryu Gumma Japan 3760051 u, Gumma 3760051, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 8, volume: 65, anno: 2001,
pagine: 1928 - 1931
SICI:
0916-8451(200108)65:8<1928:ATSFAE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC-TRANSFORMATION; RESISTANCE; FUNGUS; DNA;
Keywords:
Lyophyllum shimeji; transformation; ectomycorrhizal basidiomycete; hygromycin B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Shinozawa, T Gunma Univ, Fac Engn, Dept Biol & Chem Engn, Kiryu, Gumma 3768515, Japan Gunma Univ Kiryu Gumma Japan 3768515 u, Gumma 3768515, Japan
Citazione:
T. Saito et al., "A transformation system for an ectomycorrhizal basidiomycete, Lyophyllum shimeji", BIOS BIOT B, 65(8), 2001, pp. 1928-1931

Abstract

A transformation vector, pLS-hph, was constructed with the promoter and terminator of the glyceraldehyde-3-phosphate dehydrogenase (GPD) gene derivedfrom an ectomycorrhizal basidiomycete, Lyophyllum shimeji, and with the hygromycin B (HmB) phosphotransferase (hph) gene from Escherichia coli. This vector was introduced into protoplasts of L. shimeji and 3.4 transformants per mug plasmid DNA were obtained. In most of the transformants, multiple copies of the vector were integrated into the genomic DNA. The results indicate that pLS-hph is a useful vector for L. shimeji.

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Documento generato il 05/12/20 alle ore 01:18:26