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Titolo:
Apoptosis induction by T-2 toxin: Activation of caspase-9, caspase-3, and DFF-40/CAD through cytosolic release of cytochrome c in HL-60 cells
Autore:
Nagase, M; Alam, MM; Tsushima, A; Yoshizawa, T; Sakato, N;
Indirizzi:
Kagawa Univ, Fac Agr, Dept Life Sci, Miki, Kagawa 7610795, Japan Kagawa Univ Miki Kagawa Japan 7610795 fe Sci, Miki, Kagawa 7610795, Japan Kagawa Univ, Fac Agr, Dept Biochem & Food Sci, Miki, Kagawa 7610795, JapanKagawa Univ Miki Kagawa Japan 7610795 od Sci, Miki, Kagawa 7610795, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 8, volume: 65, anno: 2001,
pagine: 1741 - 1747
SICI:
0916-8451(200108)65:8<1741:AIBTTA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-TERMINAL KINASE; TRICHOTHECENE MYCOTOXINS; IN-VIVO; LIPID-PEROXIDATION; DNA FRAGMENTATION; TNF RECEPTOR-1; PROTEIN; MICE; ASSOCIATION; FUSARIUM;
Keywords:
trichothecene mycotoxins; apoptosis; caspase-3; DFF-45/ICAD; cytochrome c;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Nagase, M Kagawa Univ, Fac Agr, Dept Life Sci, 2393 Ikenobe, Miki, Kagawa 7610795, Japan Kagawa Univ 2393 Ikenobe Miki Kagawa Japan 7610795 610795, Japan
Citazione:
M. Nagase et al., "Apoptosis induction by T-2 toxin: Activation of caspase-9, caspase-3, and DFF-40/CAD through cytosolic release of cytochrome c in HL-60 cells", BIOS BIOT B, 65(8), 2001, pp. 1741-1747

Abstract

The molecules participating in apoptosis induced by T-2 toxin in human leukemia HL-60 cells were investigated. The rank order of the potency of trichothecene mycotoxins to induce internucleosomal DNA fragmentation was found to be T-2, satratoxin G, roridin A>>diacetoxyscirpenol > baccharin B-5>>nivalenol, deoxynivalenol, 3-acetyldeoxynivalenol, fusarenon-X, baccharin B-4=vehicle control. Western blot analysis of caspase-3 in T-2-treated cells clearly indicated the appearance of its catalytically active fragment of 17-kDa. Increased caspase-3 activity was also detected by using a fluorogenic substrate, DEVD-AMC. Next, cells exposed to T-2 led to cleavage of PARP fromits native 116-kDa form to the 85-kDa product. Moreover, DFF-45/ICAD were cleaved to give a 12.5-kDa fragment via T-2 treatment. T-2 caused the release of cytochrome c from mitochondria into the cytosol. Increased enzymic activity of caspase-9 on LEHD-AMC was shown. These data indicate that T-2-induced apoptosis involves activation of caspase-3 and DFF-40/CAD through cytosolic accumulation of cytochrome c along with caspase-9 activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:26:10