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Titolo:
Peptide chemical ligation inside living cells: In vivo generation of a circular protein domain
Autore:
Camarero, JA; Fushman, D; Cowburn, D; Muir, TW;
Indirizzi:
Rockefeller Univ, Lab Synthet Prot Chem, New York, NY 10021 USA Rockefeller Univ New York NY USA 10021 Prot Chem, New York, NY 10021 USA Rockefeller Univ, Lab Phys Biochem, New York, NY 10021 USA Rockefeller Univ New York NY USA 10021 ys Biochem, New York, NY 10021 USA
Titolo Testata:
BIOORGANIC & MEDICINAL CHEMISTRY
fascicolo: 9, volume: 9, anno: 2001,
pagine: 2479 - 2484
SICI:
0968-0896(200109)9:9<2479:PCLILC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC-PEPTIDES; BIOLOGICAL-ACTIVITY; SPLIT INTEIN; DNAE GENE; CYCLIZATION; PCC6803; LENGTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Muir, TW Rockefeller Univ, Lab Synthet Prot Chem, 1230 York Ave, New York,NY 10021USA Rockefeller Univ 1230 York Ave New York NY USA 10021 NY 10021USA
Citazione:
J.A. Camarero et al., "Peptide chemical ligation inside living cells: In vivo generation of a circular protein domain", BIO MED CH, 9(9), 2001, pp. 2479-2484

Abstract

Here we describe the first example of a peptide chemical ligation reactionperformed inside a living cell. A cell-based native chemical ligation approach was developed and used to generate a circular version of the N-terminal Src homology 3 (SH3) domain from the murine c-Crk adapter protein inside Escherichia coli cells. The in vivo cyclization reaction was extremely efficient and the resulting circular protein domain was fully biologically active and able to adopt the native SH3 folded structure. This work represents an important step towards the in vivo generation of small backbone cyclic peptides for use in basic biological research. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:57:57