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Titolo:
Analytical method of chondroitin/dermatan sulfates using high performance liquid chromatography/turbo ionspray ionization mass spectrometry: application to analyses of the tumor tissue sections on glass slides
Autore:
Oguma, T; Toyoda, H; Toida, T; Imanari, T;
Indirizzi:
Chiba Univ, Fac Pharmaceut Sci, Chiba 260, Japan Chiba Univ Chiba Japan 260 ba Univ, Fac Pharmaceut Sci, Chiba 260, Japan
Titolo Testata:
BIOMEDICAL CHROMATOGRAPHY
fascicolo: 5, volume: 15, anno: 2001,
pagine: 356 - 362
SICI:
0269-3879(200108)15:5<356:AMOCSU>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNSATURATED DISACCHARIDES; HYALURONIC-ACID; DERMATAN SULFATE; ENZYMATIC DIGESTION; GLYCOSAMINOGLYCANS; OLIGOSACCHARIDES; ISOMERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Oguma, T Daiichi Pharmaceut Co Ltd, Drug Metab & Analyt Chem Res Lab, Edogawa Ku, 1-16-13 Kitakasai, Tokyo 1348360, Japan Daiichi Pharmaceut Co Ltd 1-16-13 Kitakasai Tokyo Japan 1348360
Citazione:
T. Oguma et al., "Analytical method of chondroitin/dermatan sulfates using high performance liquid chromatography/turbo ionspray ionization mass spectrometry: application to analyses of the tumor tissue sections on glass slides", BIOMED CHRO, 15(5), 2001, pp. 356-362

Abstract

We established a highly sensitive quantitative analytical method for chondroitin/dermatan sulfates by LC/MS method. By this method, the unsaturated disaccharides produced after the enzymatic digestion of chondroitin/dermatansulfates can be determined in the amounts as low as 0.5 pmol levels. The use of tetrabutylammonium hydroxide as an ion-pair reagent for LC/MS allowedus to separate unsaturated 4-sulfated disaccharide and unsaturated 6-sulfated disaccharide. Furthermore, the peak areas of unsaturated disaccharides were increased almost 10 times by the postcolumn addition of acetonitrile. We applied this LC/MS method to the analyses of unsaturated disaccharides from chondroitin/dermatan sulfates in the tissues sections on glass slides, which were prepared from MethA tumor-bearing mice. This method brought about considerable reduction in the time distance from sample collection to preparation of analytical results. Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 09/07/20 alle ore 16:57:09