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Titolo:
Analysis of the interaction between lectins and tetra- and tri-saccharide mimics of the Sd(a) determinant by surface plasmon resonance detection
Autore:
Blanco, JLJ; Haseley, SR; Kamerling, JP; Vliegenthart, JFG;
Indirizzi:
Univ Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, NL-3508 TB Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TB 3508 TB Utrecht, Netherlands
Titolo Testata:
BIOCHIMIE
fascicolo: 7, volume: 83, anno: 2001,
pagine: 653 - 658
SICI:
0300-9084(200107)83:7<653:AOTIBL>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAMM-HORSFALL GLYCOPROTEIN; WHEAT-GERM-AGGLUTININ; CARBOHYDRATE-BINDING-SPECIFICITY; SIALIC-ACID; N-ACETYLLACTOSAMINE; DOLICHOS-BIFLORUS; MAACKIA-AMURENSIS; CELL-BINDING;
Keywords:
carbohydrate-lectin binding; Sd(a) determinant; surface plasmon resonance; Tamm-Horsfall glycoprotein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Vliegenthart, JFG Univ Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, POB 80075, NL-3508 TB Utrecht, Netherlands Univ Utrecht POB 80075 Utrecht Netherlands NL-3508 TB s
Citazione:
J.L.J. Blanco et al., "Analysis of the interaction between lectins and tetra- and tri-saccharide mimics of the Sd(a) determinant by surface plasmon resonance detection", BIOCHIMIE, 83(7), 2001, pp. 653-658

Abstract

The binding properties of a spacer-linked synthetic Sd(a) tetrasaccharide beta -D-GalpNAc-(1-->4)-alpha -Neu5Ac-(2-->3)]-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (1), two tetrasaccharide mimics beta -D-Galp-(1-->4)-alpha -Neu5Ac-(2-->3)]-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5) -NH2 (2) and beta -D-GlcpNAc-(1-->4)-alpha -Neu5Ac-(2-->3)]-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (3), and two trisaccharide mimics beta -D-GalpNAc-(1-->4)-3-O-(SO3H)-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (4) and beta -D-GalpNAc-(1-->4)-3-O-(CH2COOH)-beta -D-Galp-(1-->4)-beta -D-GlcpNAc-(1-->O)-(CH2)(5)-NH2 (5) with lectinsfrom Dolichos biflorus (DBL), Maackia amurensis (MAL), Phaseolus limensis (PLL), Ptilota plumosa (PPL), Ricinus communis 120 (RCL120) and Triticum vulgaris (wheat germ agglutinin, WGA) have been investigated by surface plasmon resonance (SPR) detection. MAL, PPL, RCL120 and WGA did not display any binding activity with compounds 1-5. However, DBL and PLL, both exhibiting GalNAc-specificity, showed strong binding activity with compounds 1, 4 and 5, and 1, 3, 4 and 5, respectively. The results demonstrate that SPR is a very useful analysis system for identifying biologically relevant oligosaccharide mimics of the Sd a determinant. (C) 2001 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS. All rights reserved.

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Documento generato il 05/12/20 alle ore 18:56:05