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Titolo: Occurrence of poly-N-acetyllactosamine synthesis in Sf-9 cells upon transfection of individual human beta-1,4-galactosyltransferase I, II, III, IV, Vand VI cDNAs
Autore: Sato, T; Guo, SC; Furukawa, K;
- Indirizzi:
- Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 okyo 1730015, Japan Beijing Med Univ, Dept Pathol, Beijing 100083, Peoples R China Beijing MedUniv Beijing Peoples R China 100083 100083, Peoples R China
- Titolo Testata:
- BIOCHIMIE
fascicolo: 8,
volume: 83,
anno: 2001,
pagine: 719 - 725
- SICI:
- 0300-9084(200108)83:8<719:OOPSIS>2.0.ZU;2-I
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- BETA-4-GALACTOSYLTRANSFERASE GENE FAMILY; ASPARAGINE-LINKED OLIGOSACCHARIDES; HAMSTER-KIDNEY CELLS; INSECT CELLS; UDP-GALACTOSE; MAMMALIAN BETA-1,4-GALACTOSYLTRANSFERASE; ACETYLGLUCOSAMINIDASES CII; EXTENSION ENZYME; SUGAR CHAINS; O-GLYCANS;
- Keywords:
- beta-1,4-galactosyltransferases I-VI; poly-N-acetyllactosamine; Sf-9 cells;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Life Sciences
- Citazioni:
- 49
- Recensione:
- Indirizzi per estratti:
- Indirizzo: Furukawa, K Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, ItabashiKu, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 , Japan
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- Citazione:
- T. Sato et al., "Occurrence of poly-N-acetyllactosamine synthesis in Sf-9 cells upon transfection of individual human beta-1,4-galactosyltransferase I, II, III, IV, Vand VI cDNAs", BIOCHIMIE, 83(8), 2001, pp. 719-725
Abstract
Lectin blot analysis of membrane glycoprotein samples from Sf-9 cells upontransfection of individual human beta -1,4-galactosyltransferase (beta -1,4-GalT) I, II, III, IV, V et VI cDNAs showed that the endogenous N-linked oligosaccharides are galactosylated (Guo et al., Glycobiology (2001), in press). Further analysis revealed that membrane glycoprotein samples from all the gene-transfected cells are also reactive to Lycopersicon esculentum agglutinin (LEA) et Datura stramonium agglutinin (DSA), both of which bind to oligosaccharides with poly-N-acetyllactosamine chains while no lectin reactive protein bands are detected when blots are pretreated with a mixture of diplococcal beta -1,4-galactosidase et jack bean beta -N-acetylhexosaminidase or N-glycanase. Analysis of endo-beta -galactosidase-digestion products revealed the presence of the Gall --> GlcNAcl --> Gal and/or GlcNAcl --> Gal structures in the gene-transfected cells. When the homogenates of the gene-transfected cells were used as enzyme sources towards oligosaccharides with the GlcNAcP1 --> (3Gal beta1 --> 4GlcNAc)(1-3) structures, human recombinant beta -1,4-GalTs I et II galactosylated these oligosaccharides more effectively than other beta -1,4-GalTs. These results indicate that beta -1,4-GalTs I-VI can synthesize poly-N-acetyllactosamine chains with beta -1,3-N-acetylglucosaminyltransferase. (C) 2001 Societe francaise de biochimie et biologie moleculaire/Editions scientifiques et medicales Elsevier SAS. All rights reserved.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:55:58