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Titolo:
Control of membrane fusion dynamics during regulated exocytosis
Autore:
Burgoyne, RD; Fisher, RJ; Graham, ME; Haynes, LP; Morgan, A;
Indirizzi:
Univ Liverpool, Physiol Lab, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England
Titolo Testata:
BIOCHEMICAL SOCIETY TRANSACTIONS
, volume: 29, anno: 2001,
parte:, 4
pagine: 467 - 472
SICI:
0300-5127(200108)29:<467:COMFDD>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENAL CHROMAFFIN CELLS; PROTEIN-KINASE-C; CYSTEINE-STRING PROTEIN; KISS-AND-RUN; NEUROTRANSMITTER RELEASE; VESICLE FUSION; SECRETORY PRODUCTS; ALPHA-SNAP; SYNTAXIN; MECHANISMS;
Keywords:
Csp; Munc 18; Noc2; protein kinase C; Rab3; SNARE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Burgoyne, RD Univ Liverpool, Physiol Lab, Crown St, Liverpool L69 3BX, Merseyside, England Univ Liverpool Crown St Liverpool Merseyside England L69 3BX
Citazione:
R.D. Burgoyne et al., "Control of membrane fusion dynamics during regulated exocytosis", BIOCH SOC T, 29, 2001, pp. 467-472

Abstract

The study of regulated exocytosis uniquely allows the direct measurement of intracellular membrane fusion events in real time. We have exploited thisto examine factors that regulate not only the extent but also the dynamicsof single fusion/release events. The general strategy used has been to assess exocytosis in transiently transfected PC12 or adrenal chromaffin cells. We aimed to design mutant constructs based on in vitro biochemistry in some cases informed by knowledge of protein structure. Using this approach we have demonstrated an inhibitory role for the putative Rab3 effector Noc2 that requires interaction with Rab3. Using carbon-fibre amperometry on adrenal chromaffin cells, we have demonstrated regulation of the kinetics of single granule release events consistent with changes in fusion pore dynamics and switches between full fusion and 'kiss-and-run' fusion. These studies have demonstrated a late role for cysteine string protein in exocytosis. In addition, they have focused attention on a key role for Munc18 in the regulation of post-fusion events that affect fusion pore dynamics.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:19:50