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Titolo:
Colony-stimulating factor-1 (CSF-1) receptor-mediated macrophage differentiation in myeloid cells: a role for tyrosine 559-dependent protein phosphatase 2A (PP2A) activity
Autore:
McMahon, KA; Wilson, NJ; Marks, DC; Beecroft, TL; Whitty, GA; Hamilton, JA; Csar, XF;
Indirizzi:
Univ Melbourne, Royal Melbourne Hosp, Dept Med, Arthrit & Inflammat Res Ctr, Parkville, Vic 3050, Australia Univ Melbourne Parkville Vic Australia 3050 arkville, Vic 3050, Australia Royal Melbourne Hosp, Cooperat Res Ctr Chron Inflammatory Dis, Parkville, Vic 3050, Australia Royal Melbourne Hosp Parkville Vic Australia 3050 le, Vic 3050, Australia
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 358, anno: 2001,
parte:, 2
pagine: 431 - 436
SICI:
0264-6021(20010901)358:<431:CF(RMD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKEMIA INHIBITORY FACTOR; MONOCYTIC DIFFERENTIATION; KINASE ACTIVATION; ESTROGEN-RECEPTOR; GROWTH ARREST; OKADAIC ACID; EXPRESSION; APOPTOSIS; PATHWAY; PHOSPHORYLATION;
Keywords:
c-Fms; ERK; okadaic acid; receptor tyrosine kinase; signalling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: McMahon, KA Univ Melbourne, Royal Melbourne Hosp, Dept Med, Arthrit & Inflammat Res Ctr, Parkville, Vic 3050, Australia Univ Melbourne Parkville VicAustralia 3050 c 3050, Australia
Citazione:
K.A. McMahon et al., "Colony-stimulating factor-1 (CSF-1) receptor-mediated macrophage differentiation in myeloid cells: a role for tyrosine 559-dependent protein phosphatase 2A (PP2A) activity", BIOCHEM J, 358, 2001, pp. 431-436

Abstract

M1 myeloid cells transfected with the wild-type (WT) colony-stimulating factor-1 (CSF-1) receptor (CSF-1R; M1/WT cells) undergo CSF-1-dependent macrophage differentiation. By mutation studies, we have provided prior evidencethat tyrosine 559 in the CSF-1R cytoplasmic domain governs the Src-dependent differentiation pathway. Further components of this pathway were then sought. We report that the extent of CSF-1-mediated tyrosine phosphorylation of protein phosphatase 2A (PP2A), and the associated loss of its activity were reduced in MI cells transfected with the CSF-IR with a tyrosine-to-phenylalanine mutation at position 559 (M1/559 cells), compared with the corresponding responses in CSF-1-treated M1/WT cells. This evidence for an involvement of a reduction in PP2A activity in the differentiation process was supported by the restoration of the defect in the CSF-1-mediated differentiation of M1/559 cells by the addition of the PP2A inhibitor, okadaic acid. Itwas also found that the degree of activation of extracellular-signal-regulated kinase (ERK) activities by CSF-1 was reduced in M1/559 cells, suggesting their involvement in the differentiation process. These data suggest that PP2A and ERK form part of the Src-dependent signal-transduction cascade governing CSF-1-mediated macrophage differentiation in M1 cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 16:45:23