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Titolo:
Transmisson of high-risk HLA-DQB1 alleles in Chilean type 1 diabetic patients and their parents: Stratification by the presence of ICA or GAD65 autoantibodies
Autore:
Perez-Bravo, F; Santos, JL; Carrasco, E; Calvillan, M; Albala, C; Puig-Domingo, M; Piquer, S; De Leiva, A;
Indirizzi:
Univ Chile, Inst Nutr & Tecnol Alimentos, Mol Biol Lab, Publ Nutr Unit, Santiago 11, Chile Univ Chile Santiago Chile 11 iol Lab, Publ Nutr Unit, Santiago 11, Chile
Titolo Testata:
AUTOIMMUNITY
fascicolo: 4, volume: 33, anno: 2001,
pagine: 285 - 291
SICI:
0891-6934(2001)33:4<285:TOHHAI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENERAL-POPULATION; IDDM; MELLITUS; MARKERS; DISEASE; SYSTEM; DESIGN; CELLS;
Keywords:
type 1 diabetes; ICA; GAD65; HLA DQB typing; association study;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Perez-Bravo, F Univ Chile, Inst Nutr & Tecnol Alimentos, Mol Biol Lab, Publ Nutr Unit, Macul 5540, Santiago 11, Chile Univ Chile Macul 5540 SantiagoChile 11 Santiago 11, Chile
Citazione:
F. Perez-Bravo et al., "Transmisson of high-risk HLA-DQB1 alleles in Chilean type 1 diabetic patients and their parents: Stratification by the presence of ICA or GAD65 autoantibodies", AUTOIMMUN, 33(4), 2001, pp. 285-291

Abstract

Aim The purpose of this study was to assess whether the transmission of DQB1*0201 and DQB1*0302 alleles from heterozygous parents to Chilean type 1 diabetic patients depends on the presence of antibodies such as glutamic acid decarboxilase (GAD65) or Islet Cell (ICA) autoantibodies in the affected case. Material and Methods A study of incident type 1 diabetic cases and parentswas carried out in Santiago, Chile during 1997-98. The use of the case-parental design eliminates the possibility that case-controls differences are due to selection of controls whose genetic backgrounds differ systematically from those of cases. HLA-DQB1 polymorphisms were determined in cases and parents from n = 83 families using polymerase chain reaction and oligonucleotide dot-blot analysis. Detection of GAD65 antibodies was performed using a simple radio-binding asssay. Conventional ICA were detected by indirect immunofluorescence. Results Transmission disequilibrium test indicate a strong association between DQB1*0201 and DQB1*0302 and type 1 diabetes. When comparing the two subsets of families defined by having an affected child tested negative or positive for GAD65 antibodies (39 and 44 case-parent trios respectively) the probability of transmission of DQB1*0201 significantly differed between such strata (p-value = 0.025). The pattern of transmission of DQB1*201 allele was also significantly different in the two subsets of families defined by ICA-or ICA+ cases (23 and 60 trios respectively) (p-value = 0.028). No differences were found in the transmission of DQB1*0302 allele in the differentstrata defined by the autoimmunity status of the proband. Conclusion Our results reveal that DQB1*0201 allele may display distinct associations with type 1 diabetes depending on the autoimmunity to ICA and GAD65 autoantibodies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:21:38