Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Usefulness of Tc-99m MIBI SPECT in predicting multidrug resistance gene expression levels in non-small cell lung cancer - a preliminary report
Autore:
Aratani, T; Narabayashi, I; Komori, T; Utsunomiya, K; Sueyoshi, K; Tachibana, S; Sasaki, S;
Indirizzi:
Osaka Med Coll, Dept Radiol & Thorac Surg, Osaka, Japan Osaka Med Coll Osaka Japan oll, Dept Radiol & Thorac Surg, Osaka, Japan
Titolo Testata:
ANNALS OF NUCLEAR MEDICINE
fascicolo: 4, volume: 15, anno: 2001,
pagine: 313 - 319
SICI:
0914-7187(200108)15:4<313:UOTMSI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
P-GLYCOPROTEIN EXPRESSION; PROTEIN MRP GENE; MALIGNANT-TUMORS; IN-VIVO; CHEMOTHERAPY; TECHNETIUM-99M-SESTAMIBI; ACCUMULATION; GLUTATHIONE; ISONITRILE; TL-201;
Keywords:
non-small cell lung cancer; Tc-99m MIBI; MDR1; MRP1; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Aratani, T Osaka Med Coll, Dept Radiol, 2-7 Daigaku Machi, Osaka, Japan Osaka Med Coll 2-7 Daigaku Machi Osaka Japan hi, Osaka, Japan
Citazione:
T. Aratani et al., "Usefulness of Tc-99m MIBI SPECT in predicting multidrug resistance gene expression levels in non-small cell lung cancer - a preliminary report", ANN NUCL M, 15(4), 2001, pp. 313-319

Abstract

In this study we investigated whether Tc-99m hexakis 2-methoxy isobutyl isonitrile (Tc-99m MIB1) single-photon emission computed tomography (SPECT) has a correlation with the multidrug resistance (MDR1) and multidrug resistance-associated protein (MRP1) gene expression levels in non-small cell lungcancer (NSCLC). Fifteen patients with NSCLC were studied. SPECT images were obtained 15 (early) and 120 (delayed) min after injection of Tc-99m MIB1. We chose only one transverse section and set the region of interest over the tumor and out of the body. The mean counts in the tumor on early and delayed images were corrected by using those in the background and representedas Te and Td, respectively. Resected tumor specimens were frozen with liquid nitrogen and each positive control cell line was cultured. After the total ribonucleic acid (RNA) was extracted from specimens and cell lines, the complimentary deoxyribonucleic acid (cDNA) was amplified by the reverse transcription-polymerase chain reaction (RT-PCR) method. Each product was electrophoresed and fluorointensity was measured. The gene expression level wasrepresented as the ratio of that of the positive control cell line. Te andTd indicated a significant correlation with the MDR1 gene expression level(p = 0.015 and p = 0.022), but not the gene of MRP1 (p = 0.100 and p = 0.145). In conclusion, Te and Td are useful parameters in predicting the MDR1 gene expression level, but not MRP1 in NSCLC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:10:19