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Titolo:
Condensed chromatin surface and NORs surface enhancement in mitogen-stimulated lymphocytes of Down syndrome patients
Autore:
Demirtas, H; Imamoglu, N; Donmez, H; Cucer, N; Yilmaz, A; Candemir, Z;
Indirizzi:
Erciyes Univ, Fac Med, Med Biol & Genet Dept, TR-38039 Kayseri, Turkey Erciyes Univ Kayseri Turkey TR-38039 enet Dept, TR-38039 Kayseri, Turkey Erciyes Univ, Dept Physiol, Fac Med, TR-38039 Kayseri, Turkey Erciyes Univ Kayseri Turkey TR-38039 , Fac Med, TR-38039 Kayseri, Turkey
Titolo Testata:
ANNALES DE GENETIQUE
fascicolo: 2, volume: 44, anno: 2001,
pagine: 77 - 82
SICI:
0003-3995(200104/06)44:2<77:CCSANS>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOLUS ORGANIZER REGIONS; INTERPHASE NUCLEI; RIBOSOMAL GENES; ACROCENTRIC CHROMOSOMES; CELLS; POSITION; PROTEINS; DNA;
Keywords:
Down syndrome; NORs; human acrocentrics; condensed chromatin; trisomy 21;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Demirtas, H Erciyes Univ, Fac Med, Med Biol & Genet Dept, TR-38039 Kayseri, Turkey Erciyes Univ Kayseri Turkey TR-38039 R-38039 Kayseri, Turkey
Citazione:
H. Demirtas et al., "Condensed chromatin surface and NORs surface enhancement in mitogen-stimulated lymphocytes of Down syndrome patients", ANN GENET, 44(2), 2001, pp. 77-82

Abstract

Mitogen-stimulated lymphocytes of 20 Down syndrome (DS) patients with regular trisomy 21 contain more condensed chromatin surface (11.28 +/- 2.64% ofthe total nuclear surface: mean SD) and more nucleolus organiser regions surface (13.21 +/- 3.45 %) than that of 12 healthy controls: (8.84 +/- 2.23 and 9.12 +/- 2.33 %, reciprocally). The source of this peculiarity has beeninvestigated. A computer program was designed for the planimetric measurement of the condensed chromatin surface (CCs)/ total nuclear surface(TNs) and the nucleolus organiser regions surface (NORss) /TNs proportions in interphase nuclei. CCs/TNs and NORss/TNs of 100 maximally activated nuclei (MANS) were measured for each patient and control case. The difference was foundhighly significant (P < 0.01). Nuclei with a diameter of greater than or equal to 17 mum measured on the slide (in flattened state) were considered as maximally activated nuclei (MANS). NORss/TNs enhancement and fluorescent in situ hybridisation (FISH) studies in MANS of DS patients indicate that this phenomenon is due to the over-expression (or lack of downregulative mechanism) of NORs (rDNA) to some extent, including the NOR of the supernumerary chromosome 21. No statistical difference was observed between 12 healthycontrols and 5 Robertsonian translocation type of DS Patients (where the two involved NORs are missing) when the two parameters were considered. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

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Documento generato il 15/08/20 alle ore 19:07:40