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Titolo:
Characterization of recombinant sucrose synthase 1 from potato for the synthesis of sucrose analogues
Autore:
Romer, U; Nettelstroth, N; Kockenberger, W; Elling, L;
Indirizzi:
Univ Dusseldorf, Res Ctr Julich, Inst Enzyme Technol, D-52426 Julich, Germany Univ Dusseldorf Julich Germany D-52426 Technol, D-52426 Julich, Germany Univ Nottingham, Sch Phys & Astron, Magnet Resonance Ctr, Nottingham NG7 2RD, England Univ Nottingham Nottingham England NG7 2RD , Nottingham NG7 2RD, England
Titolo Testata:
ADVANCED SYNTHESIS & CATALYSIS
fascicolo: 6-7, volume: 343, anno: 2001,
pagine: 655 - 661
SICI:
1615-4150(200108)343:6-7<655:CORSS1>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE SUGARS; GLUCOSE; PLANTS; SACCHARIDES; FRUCTOSE; ION;
Keywords:
enzyme catalysis; glycosides; glycoconjugates; sucrose synthase; sucrose analogues;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Elling, L Univ Dusseldorf, Res Ctr Julich, Inst Enzyme Technol, D-52426 Julich, Germany Univ Dusseldorf Julich Germany D-52426 D-52426 Julich, Germany
Citazione:
U. Romer et al., "Characterization of recombinant sucrose synthase 1 from potato for the synthesis of sucrose analogues", ADV SYNTH C, 343(6-7), 2001, pp. 655-661

Abstract

The characteristics and the application or recombinant sucrose synthase 1 (SuSy1) from potato for the synthesis of sucrose analogues are described. With UDP-Glc as donor substrate SuSy1 accepts a variety of ketoses, e.g., 1-deoxy-1-fluoro-D-fructose (6; 100%), L-sorbose (7, 55%), and D-xylulose (8;42%), as well as aldoses, e.g., D-talose (15; 36%), Didose (16; 24%), D-lyxose (12; 48%), L-arabinose (13; 36%), and D-ribose (14; 7%). Kinetic analyses revealed that the non-natural acceptors 6 (k(cat)/ K-m = 3.5 s(-1) mM(-1)), 7 (k(cat)/K-m = 1.10(-2) s(-1) mM(-1)), 8 (k(cat)/K-m = 2.10(-2) s(-1)mM(-1)), and 12 (k(cat)/K-m = 2.10(-2) s(-1) mM-1) were relatively poor substrates when compared to D-fructose (5; k(cat)/K-m = 34.1 s(-1) mm(-1)). It is concluded that the configuration and/or presence of the hydroxymethyl group at C5 determine the affinity of the ketoses for SuSy1. The acceptanceof aldoses can be explained by their flexible chair conformations, which lead to isosteric hydroxy groups recognized by SuSyl. The preparative synthesis of sucrose analogues yielded 1 ' -deoxy-1 ' -fluoro-beta -D-fructofuranosyl-alpha -D-glucopyranoside (1), [C-13(1)]-beta -D-fructofuranosyl-alpha -D-glucopyranoside (2), alpha -D-glucopyranosyl-alpha -L-sorbofuranoside (3), and alpha -D-glucopyranosyl-alpha -D-lyxopyranoside (4), in a 0.1 - 1.0 g scale. The sucrose analogues 1, 3, and 4 were not hydrolyzed by invertase, which makes them valuable tools for studies on signal transduction pathways and sugar transport in plants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:50:25