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Titolo:
Tissue-specific and developmental-specific expression of an Arabidopsis thaliana gene encoding the lipoamide dehydrogenase component of the plastid pyruvate dehydrogenase complex
Autore:
Drea, SC; Mould, RM; Hibberd, JM; Gray, JC; Kavanagh, TA;
Indirizzi:
Univ Dublin Trinity Coll, Dept Genet, Dublin 2, Ireland Univ Dublin Trinity Coll Dublin Ireland 2 Dept Genet, Dublin 2, Ireland Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England Univ CambridgeCambridge England CB2 3EA Sci, Cambridge CB2 3EA, England
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 6, volume: 46, anno: 2001,
pagine: 705 - 715
SICI:
0167-4412(200108)46:6<705:TADEOA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY-ACID SYNTHESIS; CHLOROPLAST TRANSIT PEPTIDES; GREEN FLUORESCENT PROTEIN; PEA-LEAF MITOCHONDRIA; DIHYDROLIPOAMIDE DEHYDROGENASE; GLYCINE DECARBOXYLASE; NUCLEOTIDE-SEQUENCES; HIGHER-PLANTS; ACETYL-COA; CLONING;
Keywords:
Arabidopsis thaliana; chloroplast import; GFP fusion protein; lipoamide dehydrogenase; transit peptide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Kavanagh, TA Univ Dublin Trinity Coll, Dept Genet, Dublin 2, Ireland Univ Dublin Trinity Coll Dublin Ireland 2 Dublin 2, Ireland
Citazione:
S.C. Drea et al., "Tissue-specific and developmental-specific expression of an Arabidopsis thaliana gene encoding the lipoamide dehydrogenase component of the plastid pyruvate dehydrogenase complex", PLANT MOL B, 46(6), 2001, pp. 705-715

Abstract

We describe an Arabidopsis thaliana gene, ptlpd2, which codes for a protein with high amino acid similarity to lipoamide dehydrogenases (LPDs) from diverse species. Ptlpd2 codes for a precursor protein possessing an N-terminal extension predicted to be a plastid-targeting signal. Expression of the ptlpd2 cDNA in Escherichia coli showed the encoded protein possessed the predicted LPD activity. PTLPD2 protein, synthesized in vitro, was efficientlyimported into isolated chloroplasts of Pisum sativum and shown to be located in the stroma. In addition, fusion proteins containing the predicted transit peptide of PTLPD2 or the entire protein fused at the N-terminus with the green fluorescent protein (GFP), showed accumulation in vivo in chloroplasts but not in mitochondria of A. thaliana. Expression of ptlpd2 was investigated by introducing ptlpd2 promoter-beta -glucuronidase (GUS) gene fusions into Nicotiana tabacum. GUS expression was observed in seeds, flowers, root tips and young leaves. GUS activity was highest in mature seeds, decreased on germination and increased again in young leaves. Expression was alsofound to be temporally regulated in pollen grains where it was highest in mature grains at dehiscence. Database searches on ptlpd2 sequences identified a second A. thaliana gene encoding a putative plastidial LPD and two genes encoding proteins with high similarity to the mitochondrial LPD of P. sativum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 00:08:27