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Titolo:
Expression and phylogeny of the multiple antenna genes of the low-light-adapted strain Prochlorococcus marinus SS120 (Oxyphotobacteria)
Autore:
Garczarek, L; van der Staay, GWM; Hess, WR; Le Gall, F; Partensky, F;
Indirizzi:
CNRS, Observ Oceanol Roscoff, F-29682 Roscoff, France CNRS Roscoff France F-29682 erv Oceanol Roscoff, F-29682 Roscoff, France Univ Paris 06, Biol Stn, F-29682 Roscoff, France Univ Paris 06 Roscoff France F-29682 , Biol Stn, F-29682 Roscoff, France Humboldt Univ, Inst Biol Genet, D-10115 Berlin, Germany Humboldt Univ Berlin Germany D-10115 Biol Genet, D-10115 Berlin, Germany
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 6, volume: 46, anno: 2001,
pagine: 683 - 693
SICI:
0167-4412(200108)46:6<683:EAPOTM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORTH PACIFIC-OCEAN; 2 STRAINS; PROKARYOTE PROCHLOROCOCCUS; PHOTOSYNTHETIC PROKARYOTE; PROCHLOROTHRIX-HOLLANDICA; EVOLUTIONARY ORIGINS; SYNECHOCOCCUS SP; CHLOROPHYLL-B; PROTEINS; CYANOBACTERIA;
Keywords:
chlorophyll a/b-binding proteins; CP43; light-harvesting complexes; light-regulated gene expression; marine cyanobacteria; Pcb;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Partensky, F CNRS, Observ Oceanol Roscoff, BP 74, F-29682 Roscoff, France CNRS BP 74 Roscoff France F-29682 4, F-29682 Roscoff, France
Citazione:
L. Garczarek et al., "Expression and phylogeny of the multiple antenna genes of the low-light-adapted strain Prochlorococcus marinus SS120 (Oxyphotobacteria)", PLANT MOL B, 46(6), 2001, pp. 683-693

Abstract

In contrast to typical cyanobacteria, Prochlorococcus strains possess an intrinsic divinyl-chlorophyll (Chl) a/b-protein complex instead of phycobilisomes as the major light-harvesting system. These pigment-protein complexesare encoded by a variable number of pcb genes depending on the ecotype to which the Prochlorococcus strain belongs: low-light-adapted strains possessseveral pcb gene copies whereas only a single copy is present in high-light-adapted strains. In this study, the light-regulated expression of the seven pcb genes of Prochlorococcus marinus SS120 was examined. The pcbF gene was found to exhibit a high turnover and its mRNA could only be detected as a degraded product under all light conditions. Steady-state levels of transcripts originating from the six other pcb gene copies varied over several orders of magnitude but were not significantly differentially regulated by light intensity. Transcript levels of most pcb genes increased between 4.5 and 8.5 mu mol quanta m(-2) s(-1), peaked at 45 mu mol m(-2) s(-1) and decreased at the highest irradiance (72 mu mol m(-2) s(-1)). A phylogenetic analysis of the Pcb proteins and other members of the six-helix Chl protein superfamily revealed that PcbC and PcbG make a separate cluster with regard tothe other Pcbs from SS120. In contrast, Pcb sequences from four high-light-adapted Prochlorococcus sp. strains were found to cluster together and to be less variable than SS120 Pcbs. Thus, pcb genes likely evolved at a different rate in the two Prochlorococcus ecotypes. Their early multiplication and diversification is likely a key factor in the successful adaptation of some genotypes to very-low-light conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:06:03