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Titolo:
Molecular cloning of a novel pathogen-inducible cDNA encoding a putative acyl-CoA synthetase from Capsicum annuum L.
Autore:
Lee, SJ; Suh, MC; Kim, S; Kwon, JK; Kim, M; Paek, KH; Choi, D; Kim, BD;
Indirizzi:
Seoul Natl Univ, Coll Agr & Life Sci, Sch Plant Sci, Suwon 441744, South Korea Seoul Natl Univ Suwon South Korea 441744 Sci, Suwon 441744, South Korea Seoul Natl Univ, Ctr Plant Mol Genet & Breeding Res, Suwon 441744, South Korea Seoul Natl Univ Suwon South Korea 441744 Res, Suwon 441744, South Korea Korea Res Inst Biosci & Biotechnol, Plant Cell Biotechnol Lab, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 South Korea Korea Univ, Grad Sch Biotechnol, Plant Mol Biol & Genet Lab, Seoul 136701,South Korea Korea Univ Seoul South Korea 136701 Genet Lab, Seoul 136701,South Korea
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 6, volume: 46, anno: 2001,
pagine: 661 - 671
SICI:
0167-4412(200108)46:6<661:MCOANP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC ACQUIRED-RESISTANCE; PLANT-DISEASE RESISTANCE; TOBACCO MOSAIC-VIRUS; SALICYLIC-ACID; GENE; EXPRESSION; BIOSYNTHESIS; INDUCTION; PROTEINS; PROMOTER;
Keywords:
acyl-CoA synthetase; AMP-binding protein; Capsicum annuum L.; mRNA differential display; salicylic acid; subcellular localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Choi, D Seoul Natl Univ, Coll Agr & Life Sci, Sch Plant Sci, 103 Seodoon Dong, Suwon 441744, South Korea Seoul Natl Univ 103 Seodoon Dong Suwon South Korea 441744 h Korea
Citazione:
S.J. Lee et al., "Molecular cloning of a novel pathogen-inducible cDNA encoding a putative acyl-CoA synthetase from Capsicum annuum L.", PLANT MOL B, 46(6), 2001, pp. 661-671

Abstract

By means of differential display, a pool of salicylic acid (SA)-induced mRNAs were identified and subsequently their cDNAs were isolated from a cDNA library prepared from SA-induced leaf tissues of hot pepper. One of these cDNA clones, designated CaSIG4, was 1900 bp and contained an open reading frame encoding 523 amino acids with a calculated molecular mass of 56.3 kDa. The predicted amino acid sequence of CaSIG4 showed high sequence similarityto the AMP-binding protein family of both prokaryotic and eukaryotic acyl-CoA synthetases. CaSIG4 transcripts accumulated rapidly after SA treatment and in response to both incompatible and compatible interactions with Xanthomonas campestris pv. vesicatoria race 1. To investigate the cis-acting elements mediating CaSIG4 expression, the CaSIG4 5'-flanking region was isolated by inverse PCR. Database searches indicated that a potential cis-regulatory element is almost identical to the consensus core sequences ACC(A/T)ACC(A/C) which are conserved among promoters of other phenylpropanoid biosynthetic genes. The subcellular localization of the CaSIG4 protein was studied by using a soluble modified GFP gene fusion delivered into epidermal cells of onion by biolistic bombardment. The CaSIG4-smGFP fusion protein was localized to the plasma membrane. Taken together, CaSIG4 encoding a putative acyl-CoA synthetase could function as a plasma membrane-bound protein with a role in signaling in plant defense.

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Documento generato il 30/03/20 alle ore 18:25:08