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Titolo:
Endogenous hormone concentrations and embryogenic callus development in wheat
Autore:
Jimenez, VM; Bangerth, F;
Indirizzi:
Univ Hohenheim, Inst Obst Gemuse & Weinbau 370, D-70593 Stuttgart, GermanyUniv Hohenheim Stuttgart Germany D-70593 370, D-70593 Stuttgart, Germany
Titolo Testata:
PLANT CELL TISSUE AND ORGAN CULTURE
fascicolo: 1, volume: 67, anno: 2001,
pagine: 37 - 46
SICI:
0167-6857(200110)67:1<37:EHCAEC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRITICUM-AESTIVUM L; CULTURED IMMATURE EMBRYOS; SOMATIC EMBRYOGENESIS; PLANT-REGENERATION; ABSCISIC-ACID; LIQUID CULTURE; ENVIRONMENT; MODULATION; INDUCTION; GENOTYPE;
Keywords:
in vitro culture; phytohormones; radio-immunoassay; somatic embryogenesis; Triticum aestivum (L.);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Bangerth, F Univ Hohenheim, Inst Obst Gemuse & Weinbau 370, D-70593 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70593 Stuttgart, Germany
Citazione:
V.M. Jimenez e F. Bangerth, "Endogenous hormone concentrations and embryogenic callus development in wheat", PL CELL TIS, 67(1), 2001, pp. 37-46

Abstract

Immature zygotic embryos of two wheat (Triticum aestivum L.) genotypes, known for their different ability to generate embryogenic callus, were used as initial explants to establish callus cultures. Embryogenic and non-embryogenic calluses were obtained from the competent genotype (`Combi'), while only non-embryogenic callus was produced by the incompetent one (`Devon'). The morphogenetic competence of each callus type was evaluated by transferring some segments to regeneration conditions. The endogenous hormone concentrations (free indole-3-acetic acid [IAA], abscisic acid [ABA], gibberellins(1), (3) and (20) [GAs], zeatin/zeatin riboside [Z/ZR] and N-6[Delta (2)-isopentenyl] adenine/ N-6[Delta 2-isopentenyl] adenosine; [iP/iPA]) of the initial explants were determined by means of radio-immunoassay and showed that the only difference was the higher concentration of ABA found in the embryos of the most competent genotype; whose embryos showed a reduced rate ofprecocious germination. When analysing the endogenous hormone concentrations in the various callus types generated in each genotype, it was found that only differences in the free IAA concentrations were associated with variations in the morphogenic properties of the calluses. Higher concentrationsof endogenous free IAA were typical of embryogenic callus cultures. It wasalso observed that a loss in the embryogenic competence of the calluses, due to a prolonged time of culture, occurred concomitantly with a reduction in free IAA concentrations, practically to the concentrations found in the non-embryogenic calluses.

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Documento generato il 20/09/20 alle ore 04:37:34