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Titolo:
Plant regeneration from embryogenic suspension cultures of dune reed
Autore:
Wang, W; Cui, SX; Zhang, CL;
Indirizzi:
Lanzhou Univ, Sch Life Sci, Inst Plant Physiol, Lanzhou 730000, Peoples R China Lanzhou Univ Lanzhou Peoples R China 730000 zhou 730000, Peoples R China
Titolo Testata:
PLANT CELL TISSUE AND ORGAN CULTURE
fascicolo: 1, volume: 67, anno: 2001,
pagine: 11 - 17
SICI:
0167-6857(200110)67:1<11:PRFESC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRITICUM-AESTIVUM L; CELL-SUSPENSION; COMMUNIS; BARLEY;
Keywords:
Gramineae; in vitro culture; Phragmites communis Trinius; somatic embryogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, CL Lanzhou Univ, Sch Life Sci, Inst Plant Physiol, Lanzhou 730000, Peoples R China Lanzhou Univ Lanzhou Peoples R China 730000 0, Peoples R China
Citazione:
W. Wang et al., "Plant regeneration from embryogenic suspension cultures of dune reed", PL CELL TIS, 67(1), 2001, pp. 11-17

Abstract

Embryogenic callus, derived from mature seeds of dune reed ( Phragmites communis Trinius) was used to establish suspension culture. Green shoot-forming type and albino shoot-forming type embryogenic callus of dune reed were selected carefully by the difference of shape and color of callus growing under light and mechanically dispersed before suspending in liquid MS mediumsupplemented with 1.0 mg l(-1)2,4-D. They were subcultured every 5 days toremove mucilaginous material in the early culture stage. Both fine albino and green shoot-forming cell suspension lines of dune reed were composed ofrapidly growing small cell aggregates that were densely cytoplasmic and potentially embryogenic. Globular somatic embryos were continuously produced in each liquid medium containing 1.0 mg l(-1) 2,4-D. The cell aggregates infine albino cell suspension line (size below 300 mum) were smaller than that of green shoot-forming cell suspension line (size between 300 and 800 mum). Following transfer to a differentiation medium, both suspension cultures formed regenerating plants with normal roots and albinotic or green shoots, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:03:10