Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Efficient, long-term transgene expression in Xenopus laevis dermal melanophores
Autore:
Gatlin, J; Unett, DJ; Lerner, MR; Garcia, JV;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Internal Med, Div Infect Dis Y9 206, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 iv Infect Dis Y9 206, Dallas, TX 75390 USA Univ Texas, SW Med Ctr, Dept Dermatol, Div Infect Dis, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 atol, Div Infect Dis, Dallas, TX 75390 USA Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Ctr, Dept Pharmacol, Dallas, TX 75390 USA
Titolo Testata:
PIGMENT CELL RESEARCH
fascicolo: 4, volume: 14, anno: 2001,
pagine: 275 - 282
SICI:
0893-5785(200108)14:4<275:ELTEIX>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INACTIVATING LENTIVIRUS VECTOR; FUNCTIONAL GABA(B) RECEPTOR; PROTEIN-COUPLED RECEPTORS; GENE-TRANSFER VECTORS; CD34(+) CELLS; PIGMENT DISPERSION; FROG MELANOPHORES; TRANSDUCTION; BIOASSAY; LIGANDS;
Keywords:
melanophores; transgene expression; Xenopus laevis; lentivirus vectors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia, JV Univ Texas, SW Med Ctr, Dept Internal Med, Div Infect Dis Y9 206, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 is Y9 206, Dallas, TX 75390 USA
Citazione:
J. Gatlin et al., "Efficient, long-term transgene expression in Xenopus laevis dermal melanophores", PIGM CELL R, 14(4), 2001, pp. 275-282

Abstract

Xenopus laevis dermal melanophores provide an excellent model system for the investigation of complex cellular processes. Specifically, the expression of exogenous genes in Xenopus melanophores is the basis of recombinant bioassays for the study of receptor-ligand interactions. However, due to their slow rate of cell division and to the relatively low efficiency of current transfection protocols, long-term expression of exogenous genes and the generation of stable melanophore cell lines remains problematic. In this report we demonstrate the efficient, long-term expression of two exogenous proteins, the enhanced green fluorescent protein (EGFP) and the human CD4 (hCD4) cell surface receptor, following stable introduction into Xenopus melanophores via an HIV-1 based vector. Transduction of melanophores with the EGFP expression vector resulted in up to 80% EGFP(+) cells. After 1 year in continuous culture in the absence of antibiotic selection, more than 60% of the cells remained EGFP(+). Furthermore, we demonstrate the expression of hCD4 in melanophores for over 9 months in continuous culture in the absence of antibiotic selection. Our results indicate that lentivirus vectors provide an efficient means of introducing genetic information into Xenopus melanophores, resulting in sustained levels of gene expression. The significance of this gene transfer system for the study of cellular signal transduction pathways is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:43:32