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Titolo:
Intranephron localization and regulation of the V1a vasopressin receptor during chronic metabolic acidosis and dehydration in rats
Autore:
Tashima, Y; Kohda, Y; Nonoguchi, H; Ikebe, M; Machida, K; Star, RA; Tomita, K;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Internal Med 3, Kumamoto 8608556, Japan Kumamoto Univ Kumamoto Japan 8608556 rnal Med 3, Kumamoto 8608556, Japan Univ Texas, SW Med Ctr, Div Nephrol, Dept Med, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 iv Nephrol, Dept Med, Dallas, TX 75235 USA NIDDK, Renal Diagnost & Therapeut Unit, NIH, Bethesda, MD 20892 USA NIDDKBethesda MD USA 20892 & Therapeut Unit, NIH, Bethesda, MD 20892 USA
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 5, volume: 442, anno: 2001,
pagine: 652 - 661
SICI:
0031-6768(200108)442:5<652:ILAROT>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIN-CONVERTING ENZYME-1; CORTICAL COLLECTING DUCT; ARGININE-VASOPRESSIN; V2 RECEPTOR; MESSENGER-RNA; RENAL-FAILURE; NEPHRON; URINARY; KIDNEY; EXPRESSION;
Keywords:
RT-competitive PCR; vasopressin; acid-base balance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Nonoguchi, H Kumamoto Univ, Sch Med, Dept Internal Med 3, 1-1-1 Honjo, Kumamoto 8608556, Japan Kumamoto Univ 1-1-1 Honjo Kumamoto Japan 8608556 8556, Japan
Citazione:
Y. Tashima et al., "Intranephron localization and regulation of the V1a vasopressin receptor during chronic metabolic acidosis and dehydration in rats", PFLUG ARCH, 442(5), 2001, pp. 652-661

Abstract

The intrarenal localization and role of the V1a vasopressin receptor in body fluid homeostasis are unclear. We investigated the intranephron localization of Via receptor mRNA and protein using reverse transcription (RT)-competitive polymerase chain reaction (PCR) and immunohistochemistry with a specific polyclonal antibody. To determine whether the Via receptor is involved in the regulation of acid-base balance, we also examined the effects of acute and chronic metabolic acidosis and dehydration on V1a receptor expression. V1a mRNA was expressed most abundantly in the cortical collecting ducts (CCD) and decreased in the deeper CD. Expression in the glomeruli and thick ascending limbs was low. The immunohistochemical study revealed the presence of the V1a receptor in the glomeruli, the thick ascending limbs and the CD. Dehydration decreased Via mRNA expression in the CD. Chronic metabolic acidosis increased V1a receptor mRNA expression in the CD but decreased V2 receptor mRNA expression. Western blot analysis revealed up-regulation ofthe V1a receptor protein in chronic metabolic acidosis. Incubation of microdissected CCD or outer medullary CD (OMCD) in a low-pH (or or low-HCO3-) medium increased the levels of V1a receptor mRNA but decreased V2 receptor mRNA expression. Incubating OMCD with arginine vasopressin (AVP) and the V1areceptor antagonist (OPC21268) increased V2 receptor mRNA expression compared with incubation with AVP alone. These data suggest that V1a receptors are present primarily in the principal and intercalated cells in the CD and that these receptors are involved in the regulation of water and acid-base balance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:51:35