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Titolo:
Characterisation of a human acid-sensing ion channel (hASIC1a) endogenously expressed in HEK293 cells
Autore:
Gunthorpe, NJ; Smith, GD; Davis, JB; Randall, AD;
Indirizzi:
GlaxoSmithKline Pharmaceut, Neurosci Res, Harlow CM19 5AW, Essex, England GlaxoSmithKline Pharmaceut Harlow Essex England CM19 5AW , Essex, England
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 5, volume: 442, anno: 2001,
pagine: 668 - 674
SICI:
0031-6768(200108)442:5<668:COAHAI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
VANILLOID RECEPTOR RVR1; CAPSAICIN RECEPTOR; EVOKED CURRENTS; NEURONS; CLONING; SUBUNIT; MEMBER; FAMILY; BRAIN;
Keywords:
acidosis; capsaicin; desensitisation; nociceptor; pain; patch clamp; proton-gated ion channel; vanilloid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Gunthorpe, NJ GlaxoSmithKline Pharmaceut, Neurosci Res, New Frontiers Sci Pk N, Harlow CM19 5AW, Essex, England GlaxoSmithKline Pharmaceut New Frontiers Sci Pk N Harlow Essex England CM19 5AW
Citazione:
N.J. Gunthorpe et al., "Characterisation of a human acid-sensing ion channel (hASIC1a) endogenously expressed in HEK293 cells", PFLUG ARCH, 442(5), 2001, pp. 668-674

Abstract

Acid-sensing ion channels (ASICs) are a new and expanding family of proton-gated cation (Na+/Ca2+) channels that are widely expressed in sensory neurons and the central nervous system. Their distribution suggests that they may play a critical role in the sensation of the pain that accompanies tissue acidosis and may also be important in detecting the subtle pH variations that occur during neuronal signalling. Here, using whole-cell patch-clamp electrophysiology and reverse transcriptase-polymerase chain reaction (RT-PCR), we show that HEK293 cells, a commonly used cell line for the expressionand characterisation of many ion channels, functionally express an endogenous proton-gated conductance attributable to the activity of human ASIC1a. These data therefore represent the first functional characterisation of hASIC1 and have many important implications for the use of HEK293 cells as a host cell system for the study of ASICs, vanilloid receptor-1 and any other proton-gated channel. With this latter point in mind we have devised a simple desensitisation strategy to selectively remove the contribution of hASIC1a from proton-gated currents recorded from HEK293 cells expressing vanilloid receptor-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 23:08:47