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Titolo:
A DNA-binding peptide from a phage display library
Autore:
Wolcke, J; Weinhold, E;
Indirizzi:
Max Planck Inst Mol Physiol, Phys Biochem Abt, D-44227 Dortmund, Germany Max Planck Inst Mol Physiol Dortmund Germany D-44227 7 Dortmund, Germany
Titolo Testata:
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
fascicolo: 4-7, volume: 20, anno: 2001,
pagine: 1239 - 1241
SICI:
1525-7770(2001)20:4-7<1239:ADPFAP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFFINITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
5
Recensione:
Indirizzi per estratti:
Indirizzo: Weinhold, E Max Planck Inst Mol Physiol, Phys Biochem Abt, Otto Hahn Str 11, D-44227 Dortmund, Germany Max Planck Inst Mol Physiol Otto Hahn Str 11 Dortmund Germany D-44227
Citazione:
J. Wolcke e E. Weinhold, "A DNA-binding peptide from a phage display library", NUCLEOS NUC, 20(4-7), 2001, pp. 1239-1241

Abstract

A DNA-binding peptide was selected from a random peptide phage display library. For competitive elution using the DNA methyltransferase M . TaqI in the selection step, a biotin-labeled duplex oligodeoxyribonucleotide containing the 5'-TCGA-3' recognition sequence of M . TaqI was employed. Nine of ten phages selected were found to have the same deduced amino acid sequence SVSVGMKPSPRP. The selected phage binds to DNA, as demonstrated in an ELISA.

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Documento generato il 04/04/20 alle ore 08:28:39