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Titolo:
Visualizing postendocytic traffic of synaptic vesicles at hippocampal synapses
Autore:
Li, ZY; Murthy, VN;
Indirizzi:
Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA Harvard Univ Cambridge MA USA 02138 ellular Biol, Cambridge, MA 02138 USA
Titolo Testata:
NEURON
fascicolo: 4, volume: 31, anno: 2001,
pagine: 593 - 605
SICI:
0896-6273(20010830)31:4<593:VPTOSV>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FROG NEUROMUSCULAR-JUNCTION; CLATHRIN-MEDIATED ENDOCYTOSIS; GREEN FLUORESCENT PROTEINS; NEUROTRANSMITTER RELEASE; ACTIVE ZONES; TRANSMITTER RELEASE; DEPENDENT MECHANISM; MEMBRANE RETRIEVAL; NERVE-TERMINALS; MOTOR PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Murthy, VN Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USAHarvard Univ Cambridge MA USA 02138 l, Cambridge, MA 02138 USA
Citazione:
Z.Y. Li e V.N. Murthy, "Visualizing postendocytic traffic of synaptic vesicles at hippocampal synapses", NEURON, 31(4), 2001, pp. 593-605

Abstract

We have investigated mechanisms in postendocytic, processing of synaptic vesicles at hippocampal synapses, using synaptobrevin/vesicle-associated membrane protein (VAMP) tagged with variants of the green fluorescent protein. Following exocytosis, VAMP is retrieved at synaptic and adjoining axonal regions. Retrieved VAMP-containing vesicles return to synaptic vesicle clusters at a rate slower than endocytosis. Vesicles containing a different protein, synaptophysin, recluster at a similar rate, suggesting common vesicular intermediates for the two proteins. Activity prolongs the time taken by endocytosed vesicles to return to synapses. Exogenous calcium buffers slow endocytosis but have no additional effect on the time course of reclustering. In contrast, the protein kinase inhibitor staurosporine does not affect endocytosis but slows reclustering. Finally, since VAMP can move freely on surface membranes, sustained synaptic activity leads to mixing of this vesicular component between adjacent synapses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:00:21