Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Munc18-1 promotes large dense-core vesicle docking
Autore:
Voets, T; Toonen, RF; Brian, EC; de Wit, H; Moser, T; Rettig, J; Sudhof, TC; Neher, E; Verhage, M;
Indirizzi:
Max Planck Inst Biophys Chem, Dept Membrane Biophys, D-37077 Gottingen, Germany Max Planck Inst Biophys Chem Gottingen Germany D-37077 ottingen, Germany Univ Gottingen, Sch Med, Dept Otolaryngol, D-37073 Gottingen, Germany UnivGottingen Gottingen Germany D-37073 gol, D-37073 Gottingen, Germany Univ Utrecht, Med Ctr, Mol Neurosci Rudolf Magnus Inst, NL-3584 CG Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CG 3584 CG Utrecht, Netherlands Univ Texas, SW Med Ctr, Dept Mol Genet, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Ctr, Dept Mol Genet, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Ctr Basic Neurosci, Howard Hughes Med Inst, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 ward Hughes Med Inst, Dallas, TX 75235 USA
Titolo Testata:
NEURON
fascicolo: 4, volume: 31, anno: 2001,
pagine: 581 - 591
SICI:
0896-6273(20010830)31:4<581:MPLDVD>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE ADRENAL SLICES; CHROMAFFIN CELLS; NEUROTRANSMITTER RELEASE; MEMBRANE-FUSION; REGULATED EXOCYTOSIS; TRANSMITTER RELEASE; PC12 CELLS; COMPLEX; SECRETION; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Voets, T Katholieke Univ Leuven, Fysiol Lab, Campus Gasthuisberg O&N, B-3000 Louvain, Belgium Katholieke Univ Leuven Campus Gasthuisberg O&N LouvainBelgium B-3000
Citazione:
T. Voets et al., "Munc18-1 promotes large dense-core vesicle docking", NEURON, 31(4), 2001, pp. 581-591

Abstract

Secretory vesicles dock at the plasma membrane before Ca2+ triggers their exocytosis. Exocytosis requires the assembly of SNARE complexes formed by the vesicle protein Synaptobrevin and the membrane proteins Syntaxin-1 and SNAP-25. We analyzed the role of Munc18-1, a cytosolic binding partner of Syntaxin-1, in large dense-core vesicle (LDCV) secretion. Calcium-dependent LDCV exocytosis was reduced 10-fold in mouse chromaffin cells lacking Munc18-1, but the kinetic properties of the remaining release, including single fusion events, were not different from controls. Concomitantly, mutant cellsdisplayed a 10-fold reduction in morphologically docked LDCVs. Moreover, acute overexpression of Munc18-1 in bovine chromaffin cells increased the amount of releasable vesicles and accelerated vesicle supply. We conclude that Munc18-1 functions upstream of SNARE complex formation and promotes LDCV docking.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:27:42