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Titolo:
VSV-G envelope glycoprotein forms complexes with plasmid DNA and MLV retrovirus-like particles in cell-free conditions and enhances DNA transfection
Autore:
Okimoto, T; Friedmann, T; Miyanohara, A;
Indirizzi:
Univ Calif San Diego, Sch Med, Ctr Mol Genet, UCSD Program Human Gene Therapy, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 herapy, La Jolla, CA 92093 USA Univ Calif San Diego, Sch Med, Dept Pediat, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 Pediat, La Jolla, CA 92093 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 3, volume: 4, anno: 2001,
pagine: 232 - 238
SICI:
1525-0016(200109)4:3<232:VEGFCW>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR STOMATITIS-VIRUS; MURINE LEUKEMIA-VIRUS; EFFICIENT GENE-TRANSFER; HIGH-TITER; G-PROTEIN; VECTORS; RECONSTITUTION; LIPOFECTION; GENERATION; LIPOSOMES;
Keywords:
gene therapy; retrovirus; viral vector; VSV-G; pseudotype; MLV; VLPs; cell-free assembly;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Friedmann, T Univ Calif San Diego, Sch Med, Ctr Mol Genet, UCSD Program Human Gene Therapy, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 lla, CA 92093 USA
Citazione:
T. Okimoto et al., "VSV-G envelope glycoprotein forms complexes with plasmid DNA and MLV retrovirus-like particles in cell-free conditions and enhances DNA transfection", MOL THER, 4(3), 2001, pp. 232-238

Abstract

We have previously shown that vesicles containing the spike glycoprotein of the vesicular stomatitis virus (VSV-G) can associate efficiently with immature, non-infectious, envelope-deficient retrovirus-like particles assembled by packaging cells to produce infectious, pseudotyped viruses in cell-free conditions in vitro. We have also previously reported that VSV-G can enhance DNA lipofection efficiency by interacting with liposomes to form fusogenic, serum-stable liposomes with enhanced transfection properties. Here, we report that VSV-G can form a complex directly with naked plasmid DNA in the absence of a lipofection reagent and can thereby enhance the transfection efficiency of the naked plasmid vector. Sucrose gradient sedimentation analysis demonstrated that VSV-G can also associate with plasmid DNA and murine leukemia virus (MLV) gag-pol particles to form ternary complexes that co-sediment with high DNA transfecting activity. The increased transfection efficiency with VSV-G was dependent on the presence of the polycation (Polybrene) in the culture medium during transfection. Enhanced transfection was abolished by a neutralizing antibody to VSV-G. These results may be useful in the study of retrovirus assembly, in the further design of hybrid DNA-based retrovirus-like vectors, and in the full in vitro, cell-free assembly of infectious virus-like particles from component parts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/02/20 alle ore 19:44:46