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Titolo:
The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum
Autore:
Patenge, N; Berendes, A; Engelhardt, H; Schuster, SC; Oesterhelt, D;
Indirizzi:
Max Planck Inst Biochem, Dept Membrane Biochem, D-82152 Martinsried, Germany Max Planck Inst Biochem Martinsried Germany D-82152 Martinsried, Germany Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany Max Planck Inst Biochem Martinsried Germany D-82152 Martinsried, Germany
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 3, volume: 41, anno: 2001,
pagine: 653 - 663
SICI:
0950-382X(200108)41:3<653:TFGCII>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE GENOME SEQUENCE; PSEUDOMONAS-AERUGINOSA; TWITCHING MOTILITY; MYXOCOCCUS-XANTHUS; METHANOCOCCUS-VOLTAE; ESCHERICHIA-COLI; MULTIGENE FAMILY; TYPE-4 FIMBRIAE; PROTEINS; ARCHAEON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Oesterhelt, D Max Planck Inst Biochem, Dept Membrane Biochem, Klopferspitz18A, D-82152 Martinsried, Germany Max Planck Inst Biochem Klopferspitz 18AMartinsried Germany D-82152
Citazione:
N. Patenge et al., "The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum", MOL MICROB, 41(3), 2001, pp. 653-663

Abstract

In this study, a flagella-related protein gene cluster is described for Halobacterium salinarum. The fla gene cluster is located upstream of the flagellin genes flgB1-3 and oriented in the opposite direction. It consists of nine open reading frames (ORFs): htpIX, a member of the halobacterial transducer protein gene family, and the genes flaD-K. The genes flaD, E, G, H, Iand J share high homologies with genes from other Archaea. Interestingly, flaK shows similarities to bacterial genes involved in the regulation of flagellar synthesis. The ORFs of flaH, flal and flaK contain sequences codingfor nucleotide binding sites. Furthermore, flal contains a motif called the bacterial type II secretion protein E signature, indicating a functional relation to members of the bacterial pili type IV-type II secretion proteinsuperfamily. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the genes flaE to flaK are transcribed into one polycistronic message. In frame deletion mutants of flal were generated by gene replacement. The deletion strain lacks motility and belongs to the fla(-) mutant class, indicating that it is deficient in flagellar biogenesis. The overall amount of flagellin protein in Delta flal cells is reduced, although transcription of the flagellin genes is unaffected. Therefore, the flal gene product is involved in the biosynthesis, transport or assembly of flagella in H. salinarum.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 14:59:56