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Titolo:
Resolution of natural groups using iterative assignment tests: an example from two species of Australian native rats (Rattus)
Autore:
Vazquez-Dominguez, E; Paetkau, D; Tucker, N; Hinten, G; Moritz, C;
Indirizzi:
So Cross Univ, Ctr Anim Conservat Genet, Lismore, NSW 2480, Australia So Cross Univ Lismore NSW Australia 2480 et, Lismore, NSW 2480, Australia Queensland Pk & Wildlife Serv, Ctr Trop Rehabil, Yungaburra, Qld 4872, Australia Queensland Pk & Wildlife Serv Yungaburra Qld Australia 4872 72, Australia Wildlife Genet Int, Edmonton, AB T6G 2V2, Canada Wildlife Genet Int Edmonton AB Canada T6G 2V2 dmonton, AB T6G 2V2, Canada Univ Queensland, Dept Zool & Entomol, St Lucia, Qld 4072, Australia Univ Queensland St Lucia Qld Australia 4072 St Lucia, Qld 4072, Australia
Titolo Testata:
MOLECULAR ECOLOGY
fascicolo: 8, volume: 10, anno: 2001,
pagine: 2069 - 2078
SICI:
0962-1083(200108)10:8<2069:RONGUI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
POPULATION-STRUCTURE; MICROSATELLITE ANALYSIS; GENETIC-DISTANCE; CONSERVATION; DIFFERENTIATION; STATISTICS; GENOTYPES; DISPERSAL; COMPLEX; REGION;
Keywords:
iterative assignment; microsatellites; Rattus fuscipes; Rattus leucopus; species identification; wet tropics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Vazquez-Dominguez, E Univ Nacl Autonoma Mexico, Inst Ecol, Circuito Interior,Ap Postal 70-275, Mexico City 04510, DF, Mexico Univ Nacl Autonoma Mexico Circuito Interior,Ap Postal 70-275 Mexico City DF Mexico 04510
Citazione:
E. Vazquez-Dominguez et al., "Resolution of natural groups using iterative assignment tests: an example from two species of Australian native rats (Rattus)", MOL ECOL, 10(8), 2001, pp. 2069-2078

Abstract

Sympatric individuals of Rattus fuscipes and Rattus leucopus, two Australian native rats from the tropical wet forests of north Queensland, are difficult to distinguish morphologically and are often confused in the field. When we started a study on fine-scale movements of these species, using microsatellite markers, we found that the species as identified in the field didnot form coherent genetic groups. In this study, we examined the potentialof an iterative process of genetic assignment to separate specimens from distinct (e.g. species, populations) natural groups. Five loci with extensive overlap in allele distributions between species were used for the iterative process. Samples were randomly distributed into two starting groups of equal size and then subjected to the test. At each iteration, misassigned samples switched groups, and the output groups from a given round of assignment formed the input groups for the next round. All samples were assigned correctly on the 10th iteration, in which two genetic groups were clearly separated. Mitochondrial DNA sequences were obtained from samples from each genetic group identified by assignment, together with those of museum voucherspecimens, to assess which species corresponded to which genetic group. The iterative procedure was also used to resolve groups within species, adequately separating the genetically identified R. leucopus from our two sampling sites. These results show that the iterative assignment process can correctly differentiate samples into their appropriate natural groups when diagnostic genetic markers are not available, which allowed us to resolve accurately the two R. leucopus and R. fuscipes species. Our approach provides ananalytical tool that may be applicable to a broad variety of situations where genetic groups need to be resolved.

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Documento generato il 07/07/20 alle ore 12:27:23