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Titolo:
Nuclear relocalization of the pre-mRNA splicing factor PSF during apoptosis involves hyperphosphorylation, masking of antigenic epitopes, and changesin protein interactions
Autore:
Shav-Tal, Y; Cohen, M; Lapter, S; Dye, B; Patton, JG; Vandekerckhove, J; Zipori, D;
Indirizzi:
Weizmann Inst Sci, Dept Mol Cell Biol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 Biol, IL-76100 Rehovot, Israel Vanderbilt Univ, Dept Mol Biol, Nashville, TN 37235 USA Vanderbilt Univ Nashville TN USA 37235 Mol Biol, Nashville, TN 37235 USA State Univ Ghent, VIB, Dept Med Prot Chem, Ghent, Belgium State Univ Ghent Ghent Belgium VIB, Dept Med Prot Chem, Ghent, Belgium
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 8, volume: 12, anno: 2001,
pagine: 2328 - 2340
SICI:
1059-1524(200108)12:8<2328:NROTPS>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-TOPOISOMERASE-I; MESSENGER-RNA; SR PROTEINS; CELL-DEATH; INTRANUCLEAR DISTRIBUTION; TRANSCRIPTIONAL ACTIVITY; MAMMALIAN-CELLS; LIVING CELLS; SERINE-RICH; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Zipori, D Weizmann Inst Sci, Dept Mol Cell Biol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 6100 Rehovot, Israel
Citazione:
Y. Shav-Tal et al., "Nuclear relocalization of the pre-mRNA splicing factor PSF during apoptosis involves hyperphosphorylation, masking of antigenic epitopes, and changesin protein interactions", MOL BIOL CE, 12(8), 2001, pp. 2328-2340

Abstract

The spatial nuclear organization of regulatory proteins often reflects their functional state. PSF, a factor essential for pre-mRNA splicing, is visualized by the B92 mAb as discrete nuclear foci, which disappeared during apoptosis. Because this mode of cell death entails protein degradation, it was considered that PSF, which like other splicing factors is sensitive to proteolysis, might be degraded. Nonetheless, during the apoptotic process, PSF remained intact and was N-terminally hyperphosphorylated on serine and threonine residues. Retarded gel migration profiles suggested differential phosphorylation of the molecule in mitosis vs. apoptosis and under-phosphorylation during blockage of cells at G1/S. Experiments with the use of recombinant GFP-tagged PSF provided evidence that in the course of apoptosis the antigenic epitopes of PSF are masked and that PSF reorganizes into globular nuclear structures. In apoptotic cells, PSF dissociated from PTB and bound new partners, including the U1-70K and SR proteins and therefore may acquire new functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:46:18