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Titolo:
Biogenesis of Golgi stacks in imaginal discs of Drosophila melanogaster
Autore:
Kondylis, V; Goulding, SE; Dunne, JC; Rabouille, C;
Indirizzi:
Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 3JR Midlothian, Scotland
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 8, volume: 12, anno: 2001,
pagine: 2308 - 2327
SICI:
1059-1524(200108)12:8<2308:BOGSII>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
UNFOLDED PROTEIN RESPONSE; CELL-FREE SYSTEM; ENDOPLASMIC-RETICULUM; MEMBRANE-FUSION; SECRETORY PROTEINS; MATRIX PROTEIN; NSF FUNCTION; APPARATUS; IDENTIFICATION; FRAGMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Rabouille, C Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr CellBiol, Edinburgh EH9 3JR, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 3JR cotland
Citazione:
V. Kondylis et al., "Biogenesis of Golgi stacks in imaginal discs of Drosophila melanogaster", MOL BIOL CE, 12(8), 2001, pp. 2308-2327

Abstract

We provide a detailed description of Golgi stack biogenesis that takes place in vivo during one of the morphogenetic events in the lifespan of Drosophila melanogaster. In early third-instar larvae, small clusters consisting mostly of vesicles and tubules were present in epithelial imaginal disk cells. As larvae progressed through mid- and late-third instar, these larval clusters became larger but also increasingly formed cisternae, some of whichwere stacked. In white pupae, the typical Golgi stack was observed. We show that larval clusters are Golgi stack precursors by 1) localizing various Golgi-specific markers to the larval clusters by electron and immunofluorescence confocal microscopy, 2) driving this conversion in wild-type larvae incubated at 37 degreesC for 2 h, and 3) showing that this conversion does not take place in an NSF1 mutant (comt 17). The biological significance of this conversion became clear when we found that the steroid hormone 20-hydroxyecdysone (ecdysone) is critically involved in this conversion. In its absence, Golgi stack biogenesis did not occur and the larval clusters remainedunaltered. We showed that dGM130 and sec23p expression increases approximately three- and fivefold, respectively, when discs are exposed to ecdysone in vivo and in vitro. Taken together, these results suggest that we have developed an in vivo system to study the ecdysone-triggered Golgi stack biogenesis.

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Documento generato il 29/09/20 alle ore 00:40:36