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Titolo:
A conserved mechanism of synaptogyrin localization
Autore:
Zhao, HJ; Nonet, ML;
Indirizzi:
Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 & Neurobiol, St Louis, MO 63110 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 8, volume: 12, anno: 2001,
pagine: 2275 - 2289
SICI:
1059-1524(200108)12:8<2275:ACMOSL>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNAPTIC VESICLE PROTEIN; C-ELEGANS; CAENORHABDITIS-ELEGANS; PC12 CELLS; MEMBRANE-PROTEIN; HIPPOCAMPAL-NEURONS; TARGETING SIGNAL; AXONAL-TRANSPORT; CYTOPLASMIC TAIL; SYNAPTOPHYSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
85
Recensione:
Indirizzi per estratti:
Indirizzo: Nonet, ML Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 l, St Louis, MO 63110 USA
Citazione:
H.J. Zhao e M.L. Nonet, "A conserved mechanism of synaptogyrin localization", MOL BIOL CE, 12(8), 2001, pp. 2275-2289

Abstract

We have studied the localization of synaptogyrin family members in vivo. Both native and green fluorescent protein (GFP)-tagged Caenorhabditis elegans synaptogyrin (SNG-1) are expressed in neurons and synaptically localized. Deletion and mutational analysis with the use of GFP-tagged SNG-1 has defined a 38 amino acid sequence within the C terminus of SNG-1 and a single arginine in the cytoplasmic loop between transmembrane domain 2 and 3 that are required for SNG-1 localization. These domains may represent components of signals that target synaptogyrin for endocytosis from the plasma membraneand direct synaptogyrin to synaptic vesicles, respectively. In chimeric studies, these regions were sufficient to relocalize cellugyrin, a nonneuronal form of synaptogyrin, from nonsynaptic regions such as the sensory dendrites and the cell body to synaptic vesicles. Furthermore, GFP-tagged rat synaptogyrin is synaptically localized in neurons of C. elegans and in cultured hippocampal neurons. Similarly, the C-terminal domain of rat synaptogyrinis necessary for localization in hippocampal neurons. Our study suggests that the mechanisms for synaptogyrin localization are likely to be conservedfrom C. elegans to vertebrates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:35:37