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Titolo:
Caveolin-1 expression negatively regulates cell cycle progression by inducing G(0)/G(1) arrest via a p53/p21(WAF1/Cip1)-dependent mechanism
Autore:
Galbiati, F; Volonte, D; Liu, J; Capozza, F; Frank, PG; Zhu, L; Pestell, RG; Lisanti, MP;
Indirizzi:
Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 onx, NY 10461USA Yeshiva Univ Albert Einstein Coll Med, Albert Einstein Canc Ctr, Bronx, NY10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 onx, NY10461 USA Yeshiva Univ Albert Einstein Coll Med, Albert Einstein Canc Ctr, Dept Dev,Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Albert Einstein Canc Ctr, Dept BiolMol, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 8, volume: 12, anno: 2001,
pagine: 2229 - 2244
SICI:
1059-1524(200108)12:8<2229:CENRCC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; HUMAN BREAST-CANCER; ONCOGENICALLY TRANSFORMED-CELLS; RECEPTOR TYROSINE KINASE; D7S522 LOCUS 7Q31.1; ROUS-SARCOMA VIRUS; BETA-CATENIN; IN-VIVO; GENE FAMILY; MUSCULAR-DYSTROPHY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Lisanti, MP Univ Pittsburgh, Sch Med, Dept Pharmacol, BST W,Rm 1302, Pittsburgh, PA 15261 USA Univ Pittsburgh BST W,Rm 1302 Pittsburgh PA USA 15261 5261 USA
Citazione:
F. Galbiati et al., "Caveolin-1 expression negatively regulates cell cycle progression by inducing G(0)/G(1) arrest via a p53/p21(WAF1/Cip1)-dependent mechanism", MOL BIOL CE, 12(8), 2001, pp. 2229-2244

Abstract

Caveolin-1 is a principal component of caveolae membranes in vivo. Caveolin-1 mRNA and protein expression are lost or reduced during cell transformation by activated oncogenes. Interestingly, the human caveolin-1 gene is localized to a suspected tumor suppressor locus (7q31.1). However, it remains unknown whether caveolin-1 plays any role in regulating cell cycle progression. Here, we directly demonstrate that caveolin-1 expression arrests cellsin the G(0)/G(1) phase of the cell cycle. We show that serum starvation induces up-regulation of endogenous caveolin-1 and arrests cells in the G(0)/G(1) phase of the cell cycle. Moreover, targeted down-regulation of caveolin-1 induces cells to exit the G(0)/G(1) phase. Next, we constructed a greenfluorescent protein-tagged caveolin-1 (Cav-1-GFP) to examine the effect ofcaveolin-1 expression on cell cycle regulation. We directly demonstrate that recombinant expression of Cav-1-GFP induces arrest in the G(0)/G(1) phase of the cell cycle. To examine whether caveolin-1 expression is important for modulating cell cycle progression in vivo, we expressed wild-type caveolin-1 as a transgene in mice. Analysis of primary cultures of mouse embryonic fibroblasts from caveolin-1 transgenic mice reveals that caveolin-1 induces 1) cells to exit the S phase of the cell cycle with a concomitant increase in the G(0)/G(1) population, 2) a reduction in cellular proliferation, and 3) a reduction in the DNA replication rate. Finally, we demonstrate that caveolin-1-mediated cell cycle arrest occurs through a p53/p21-dependent pathway. Taken together, our results provide the first evidence that caveolin-1 expression plays a critical role in the modulation of cell cycle progression in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:21:13