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Titolo:
The Thermus thermophilus 5S rRNA-protein complex: Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA
Autore:
Gongadze, GM; Perederina, AA; Meshcheryakov, VA; Fedorov, RV; Moskalenko, SE; Rak, AV; Serganov, AA; Shcherbakov, DV; Nikonov, SV; Garber, MB;
Indirizzi:
Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia Russian Acad Sci Pushchino Moscow Region Russia 142290 cow Region, Russia
Titolo Testata:
MOLECULAR BIOLOGY
fascicolo: 4, volume: 35, anno: 2001,
pagine: 521 - 526
SICI:
0026-8933(200107/08)35:4<521:TTT5RC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-PROTEIN; ESCHERICHIA-COLI; RNA-BINDING; SECONDARY STRUCTURE; ANGSTROM RESOLUTION; RIBONUCLEIC-ACIDS; SUBUNIT; L25; REGION; DOMAIN;
Keywords:
Thermus thermophilus; bacterial ribosome; 5S rRNA; ribosomal proteins; RNA-protein interactions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Gongadze, GM Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Region, Russia Russian Acad Sci Pushchino Moscow Region Russia 142290 ussia
Citazione:
G.M. Gongadze et al., "The Thermus thermophilus 5S rRNA-protein complex: Identification of specific binding sites for proteins L5 and L18 in the 5S rRNA", MOL BIOL, 35(4), 2001, pp. 521-526

Abstract

Three 5S rRNA-binding ribosomal proteins (L5, L18, TL5) of extremely thermophilic bacterium Thermus thermophilus have earlier been isolated. Structural analysis of their complexes with rRNA requires identification of their binding sites in the 5S rRNA. Previously, a TL5-binding site has been identified, a TL5-RNA complex crystallized, and its structure determined to 2.3 Angstrom. The sites for L5 and L18 were characterized, and two corresponding5S rRNA fragments constructed. Of these, a 34-nt fragment specifically interacted with L5, and a 55-nt fragment interacted with L5, L18, and with both proteins. The 34-nt fragment-L5 complex was crystallized; the crystals are suitable for high-resolution X-ray analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:19:49