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Titolo:
Nervous system-derived chondroitin sulfate proteoglycans regulate growth cone morphology and inhibit neurite outgrowth: A light, epifluorescence, andelectron microscopy study
Autore:
Snow, DM; Mullins, N; Hynds, DL;
Indirizzi:
Univ Kentucky, Albert B Chandler Med Ctr, Dept Anat & Neurobiol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 & Neurobiol, Lexington, KY 40536 USA
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 5, volume: 54, anno: 2001,
pagine: 273 - 286
SICI:
1059-910X(20010901)54:5<273:NSCSPR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROOT GANGLION NEURONS; DEPENDENT ADHESION MOLECULE; AMYLOID PRECURSOR PROTEIN; HUMAN NEUROBLASTOMA-CELLS; ALZHEIMERS-DISEASE; HEPARAN-SULFATE; AXONAL OUTGROWTH; SCHWANN-CELLS; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION;
Keywords:
plasticity; regeneration; guidance; laminin; tissue culture; patterned substrata;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
119
Recensione:
Indirizzi per estratti:
Indirizzo: Snow, DM Univ Kentucky, Albert B Chandler Med Ctr, Dept Anat & Neurobiol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 ol, Lexington, KY 40536 USA
Citazione:
D.M. Snow et al., "Nervous system-derived chondroitin sulfate proteoglycans regulate growth cone morphology and inhibit neurite outgrowth: A light, epifluorescence, andelectron microscopy study", MICROSC RES, 54(5), 2001, pp. 273-286

Abstract

Proteoglycans influence aging and plasticity in the nervous system. Particularly prominent are the chondroitin sulfate proteoglycans (CSPGs), which are generally inhibitory to neurite outgrowth. During development, CSPGs facilitate normal guidance, but following nervous system injury and in diseases of aging (e.g., Alzheimer's disease), they block successful regeneration,and are associated with axon devoid regions and degenerating nerve cells. Whereas previous studies used non-nervous system sources of CSPGs, this study analyzed the morphology and behavior of sensory (dorsal root ganglia) neurons, and a human nerve cell model (SH-SY5Y neuroblastoma cells) as they contacted nervous system-derived CSPGs, using a variety of microscopy techniques. The results of these qualitative analyses show that growth cones of both nerve cell types contact CSPGs via actin-based filopodia, sample the CSPGs repeatedly without collapse, and alter their trajectory to avoid nervous system-derived CSPGs. Turning and branching are correlated with increasedfilopodial sampling, and are common to both neurons and Schwann cells. We show that CSPG expression by rat CNS astrocytes in culture is correlated with sensory neuron avoidance. Further, we show for the first time the ultrastructure of sensory growth cones at a CSPG-laminin border and reveal details of growth cone and neurite organization at this choice point. This type of detailed analysis of the response of growth cones to nervous system-derived CSPGs may lead to an understanding of CSPG function following injury andin diseases of aging, where CSPGs are likely to contribute to aberrant neurite outgrowth, failed or reduced synaptic connectivity, and/or ineffectiveplasticity. Microsc. Res. Tech. 54:273-286, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 09:57:44