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Titolo:
Nifedipine block of capacitative calcium entry in cultured human uterine smooth-muscle cells
Autore:
Young, RC; Schumann, R; Zhang, PS;
Indirizzi:
Med Univ S Carolina, Dept Obstet & Gynecol, Charleston, SC 29425 USA Med Univ S Carolina Charleston SC USA 29425 col, Charleston, SC 29425 USA
Titolo Testata:
JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION
fascicolo: 4, volume: 8, anno: 2001,
pagine: 210 - 215
SICI:
1071-5576(200107/08)8:4<210:NBOCCE>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SARCOPLASMIC-RETICULUM; CHANNELS; ACTIVATION; RECEPTORS;
Keywords:
myometrium; capacitative calcium entry; mechanisms of action of nifedipine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Young, RC Med Univ S Carolina, Dept Obstet & Gynecol, 96 Jonathan Lucas St,Suite 634, Charleston, SC 29425 USA Med Univ S Carolina 96 Jonathan Lucas St,Suite 634 Charleston SC USA 29425
Citazione:
R.C. Young et al., "Nifedipine block of capacitative calcium entry in cultured human uterine smooth-muscle cells", J SOC GYN I, 8(4), 2001, pp. 210-215

Abstract

Objective: To determine whether nifedipine inhibits capacitative calcium entry at clinically relevant concentrations using human myocytes as a model for human myometrium. Methods: Myocyte cultures were initiated from the myometrium of term pregnant women who underwent cesarean deliver. Paired cells were chosen for study. The cell of interest was stimulated by an intercellular calcium wave from the adjacent cell. In this fashion, release of sarcoplasmic reticulum (SR) calcium was accomplished with minimal disturbance of the plasma membrane and the subplasmalemmal space (SPS) of the cell studied. Depletion of the SR calcium stores by the calcium wave activated the capacitative calcium current, elevated calcium in the SPS, and activated calcium-activated potassium channels. A cell-attached patch clamp was used to monitor the outward current resulting from the calcium activation of these potassium channels. Calcium green-1 fluorescence was used to simultaneously monitor changes of thedeep cytosolic calcium concentration. Experiments were performed at varying concentration of nifedipine (0-10 mu mol/L). Results: Nifedipine reduced outward potassium currents in a dose-dependentmanner. Nifedipine at 100 nmol/L resulted in greater than a 50% reduction of outward current, indicating a significant inhibition of capacitative calcium entry at that concentration. Higher concentrations of nifedipine abolished outward current. Experiments designed to detect indirect effects of nifedipine on capacitative calcium entry were negative. Conclusions: Nifedipine block of capacitative calcium entry occurred at concentrations similar to those required to block L-type voltage-activated calcium channels. These data suggest that block of capacitative calcium entrymay be an important mechanism of action when nifedipine is clinically usedfor tocolysis of preterm labor. (J Soc gynecol Investig 2001; 8:210-5) Copyright (C) 2001 by the Society for Gynecologic Investigation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:23:11