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Titolo:
Differential regulation of starch synthesis gene expression during amyloplast development in cultured tobacco BY-2 cells
Autore:
Miyazawa, Y; Sakai, A; Kawano, S; Kuroiwa, T;
Indirizzi:
Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Sci, Dept Biol Sci, Tokyo 1130033, Japan Nara Womens Univ, Fac Sci, Dept Biol Sci, Nara 6308506, Japan Nara Womens Univ Nara Japan 6308506 , Dept Biol Sci, Nara 6308506, Japan Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 t Integrated Biosci, Tokyo 1130033, Japan
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 8, volume: 158, anno: 2001,
pagine: 1077 - 1084
SICI:
0176-1617(200108)158:8<1077:DROSSG>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE PYROPHOSPHORYLASE GENES; PLASTID DNA; POTATO; BIOSYNTHESIS; PHOSPHATE; PROGRESS; SYNTHASE; SUCROSE; PEA; IIB;
Keywords:
AGPase (ADP-gloose pyrophosphorylase); GBSS (granule-bound starch synthase); SBE (starch branching enzyme); gene expression; cycloheximide; amyloplast differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Miyazawa, Y RIKEN, Inst Phys & Chem Res, 2-1 Hirosawa, Wako, Saitama 3510198, Japan RIKEN 2-1 Hirosawa Wako Saitama Japan 3510198 a 3510198, Japan
Citazione:
Y. Miyazawa et al., "Differential regulation of starch synthesis gene expression during amyloplast development in cultured tobacco BY-2 cells", J PLANT PHY, 158(8), 2001, pp. 1077-1084

Abstract

Transfer of stationary-phase cultured tobacco (Nicotiana tabacum L. ) BY-2 cells into auxin-depleted culture medium induces amyloplast formation. Measurements and microscopic observations of starch content revealed that amyloplast formation begins 18 to 24 h after transfer to amyloplast-inducing medium, and involves morphological changes in plastics and the enlargement of starch granules. To investigate the timing and requirement for de novo protein synthesis in amyloplast development and the enhancement of starch synthesis gene expression, we added cycloheximide to cells grown in amyloplast-inducing media at various times and monitored the changes in plastid morphology, starch accumulation, and the mRNA levels of starch synthesis genes. Cycloheximide immediately inhibited both cell growth and amyloplast development. RNA gel-blot analyses revealed that cycloheximide treatment reduced theaccumulation of mRNA for the ADP-Glc pyrophosphorylase small subunit (AgpS) and granule-bound starch synthase (GBSS) mRNA levels, but did not affect the starch branching enzyme (SEE) mRNA levels. These results suggest that (i) the expression of at least one isoform of SBE is regulated differently from the expression of AgpS and GBSS at the transcriptional or post-transcriptional level, and (ii) at least one de novo synthesized protein is required in order to control the expression of AgpS and GBSS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:23:45