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Titolo:
Melatonin versus treatment against extra-hepatic bile vitamin E as protective oxidative stress after duct ligation in rats
Autore:
Montilla, P; Cruz, A; Padillo, FJ; Tunez, I; Gascon, F; Munoz, MC; Gomez, M; Pera, C;
Indirizzi:
Sch Med, Dept Biochem & Mol Biol, Cordoba 14004, Spain Sch Med Cordoba Spain 14004 ept Biochem & Mol Biol, Cordoba 14004, Spain Reina Sofia Univ Hosp, Dept Gen Surg, Cordoba, Spain Reina Sofia Univ Hosp Cordoba Spain Hosp, Dept Gen Surg, Cordoba, Spain
Titolo Testata:
JOURNAL OF PINEAL RESEARCH
fascicolo: 2, volume: 31, anno: 2001,
pagine: 138 - 144
SICI:
0742-3098(200109)31:2<138:MVTAEB>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSYL-L-METHIONINE; OXYGEN-FREE-RADICALS; BILIARY OBSTRUCTION; LIVER-INJURY; LIPID-PEROXIDATION; EXPERIMENTAL-MODEL; IN-VITRO; MITOCHONDRIA; CHOLESTASIS; ADRIAMYCIN;
Keywords:
free radicals; melatonin; obstructive jaundice; vitamin E;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Montilla, P Sch Med, Dept Biochem & Mol Biol, Avda Menendez Pidal S-N, Cordoba 14004, Spain Sch Med Avda Menendez Pidal S-N Cordoba Spain 14004 04, Spain
Citazione:
P. Montilla et al., "Melatonin versus treatment against extra-hepatic bile vitamin E as protective oxidative stress after duct ligation in rats", J PINEAL R, 31(2), 2001, pp. 138-144

Abstract

The aims of the present study were first to compare the effects of melatonin and vitamin E on the cholestasis syndrome and their protective effect onliver injury, and second, to evaluate the activity of antioxidant enzymes after treatment with these antioxidant drugs. Cholestasis was achieved in adult male Wistar rats by double ligature and section of the extra-hepatic biliary duct. Hepatic and plasma oxidative stress markers were evaluated by changes in the amount of lipid peroxides, measured as malondialdehyde (MDA)and reduced glutathione (GSH) in plasma and homogenates of hepatic tissue. Serum bilirubin, alkaline phosphatase (AP), and gamma-glutamyl-transpeptidase (GGT) were used to evaluate the severity of cholestasis, and serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were used to evaluate the hepatic injury. Both vitamin E and melatonin were administrated I day before and 7 days after bile duct ligation. Acute ligation of the bile duct was accompanied by a significant increased in MDA and a decrease in GSH levels in both plasma and liver, as well as a significantreduction in antioxidant enzymes activities. The overall analysis of both treatments showed that melatonin (500 mug/kg daily) offered significantly better protection against cholestasis and a superior protective effect on hepatic injury than did vitamin E (15 mg/kg daily). Although vitamin E treatment resulted in a reduction of parameters of oxidative stress, the results were significantly better after a much lower daily dose of melatonin. Moreover, melatonin treatment was associated with a significant recovery of antioxidative enzymes. In conclusion, the present paper demonstrates a correlation between the intensity of biliary tract obstruction and increased free radical generation, as well as a direct correlation between the level of oxidative stress and the biochemical markers of liver injury. Melatonin (at a much lower dose than vitamin E) was much more efficient than vitamin E in reducing the negative parameters of cholestasis, the degree of oxidative stress and provided a significantly greater hepato protective effect against the liver injury secondary to the acute ligation of the biliary duct.

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Documento generato il 01/12/20 alle ore 22:59:18